Protection of pigs against classical swine fever with DNA-delivered gp55

Classical swine fever virus causes significant mortality and morbidity in commercial piggeries in many countries in Europe and Asia. The protective antigen, gp55, is highly conformation-dependent and thus killed virus or bacterially produced proteins are not protective. This report demonstrates that...

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Bibliographic Details
Published in:Vaccine 2000-03, Vol.18 (18), p.1932-1938
Main Authors: Andrew, M.E, Morrissy, C.J, Lenghaus, C, Oke, P.G, Sproat, K.W, Hodgson, A.L.M, Johnson, M.A, Coupar, B.E.H
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - biosynthesis
Asia
Biological and medical sciences
Classical swine fever
Classical Swine Fever - immunology
Classical Swine Fever - prevention & control
Classical Swine Fever Virus - immunology
DNA vaccination
DNA vaccines
DNA, Viral - administration & dosage
DNA, Viral - genetics
DNA, Viral - immunology
DNA, Viral - therapeutic use
Europe
Fundamental and applied biological sciences. Psychology
glycoprotein gp55
Injections, Intramuscular
Microbiology
Neutralization Tests
pigs
Protection
Swine
swine fever virus
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - administration & dosage
Vaccines, DNA - therapeutic use
Viral Envelope Proteins - administration & dosage
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Viral Envelope Proteins - therapeutic use
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Virology
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Summary:Classical swine fever virus causes significant mortality and morbidity in commercial piggeries in many countries in Europe and Asia. The protective antigen, gp55, is highly conformation-dependent and thus killed virus or bacterially produced proteins are not protective. This report demonstrates that DNA vaccination with the gene encoding gp55 can provide protective immunity with inoculation of two doses of 25 μg DNA or a single shot of 200 μg. Furthermore, the DNA can be delivered intramuscularly or by a simple spring-loaded needleless inoculator. In addition it is shown that inoculation of the DNA at a single site conveys the same level of immunity as division of the dose between two sites.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(99)00445-4