Glial and neuronal localization of ionotropic glutamate receptor subunit-immunoreactivities in the median eminence of female rats: GluR2/3 and GluR6/7 colocalize with vimentin, not with glial fibrillary acidic protein (GFAP)

Female rat median eminence was immunostained with anti-NR1, GluR1, GluR2/3, GluR6/7, or KA2. GluR2/3- and GluR6/7-immunoreactivities were detected in cells lining the basal portion of the third ventricle. To identify these cells as tanycytes, the median eminence was dual-immunostained with glutamate...

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Bibliographic Details
Published in:Brain research 2000-03, Vol.858 (1), p.198-204
Main Author: Kawakami, Shin-ichi
Format: Article
Language:English
Subjects:
Animals
Astrocyte
Astrocytes - cytology
Astrocytes - metabolism
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Glial Fibrillary Acidic Protein - metabolism
GluK2 Kainate Receptor
GluK3 Kainate Receptor
Glutamate receptor
Hypothalamus. Hypophysis. Epiphysis. Urophysis
Immunohistochemistry
Median eminence
Median Eminence - metabolism
Median Eminence - ultrastructure
Morphology. Functional localizations
Neuroglia - cytology
Neuroglia - metabolism
Neurons - cytology
Neurons - metabolism
Rats
Rats, Wistar
Receptors, AMPA - metabolism
Receptors, Glutamate - metabolism
Receptors, Kainic Acid - metabolism
RNA, Messenger - metabolism
Tanycyte
tanycytes
Vertebrates: endocrinology
Vimentin - metabolism
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Summary:Female rat median eminence was immunostained with anti-NR1, GluR1, GluR2/3, GluR6/7, or KA2. GluR2/3- and GluR6/7-immunoreactivities were detected in cells lining the basal portion of the third ventricle. To identify these cells as tanycytes, the median eminence was dual-immunostained with glutamate receptors and glial cytoskeletal marker proteins, such as vimentin or glial fibrillary acidic protein (GFAP). Both GluR2/3 and GluR6/7 were shown to colocalize with vimentin, not with GFAP. These results suggest the potential role for tanycytes in conducting glutamate signaling.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(00)01980-6