Optimization of electrophoresis for the identification of low molecular mass allergens in hazelnuts

Conventional electrophoresis techniques used to identify food allergens are insufficient to separate low molecular mass proteins and peptides. In this paper we performed three different methods which provided an extended resolving power for small proteins. Applying the improved techniques, we were a...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-05, Vol.756 (1), p.105-111
Main Authors: Schocker, Frauke, Becker, Wolf-Meinhard
Format: Article
Language:English
Subjects:
Allergens - chemistry
Electrophoresis, Polyacrylamide Gel
Female
Food Hypersensitivity
Humans
Low molecular mass allergens
Molecular Weight
Nuts - chemistry
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Summary:Conventional electrophoresis techniques used to identify food allergens are insufficient to separate low molecular mass proteins and peptides. In this paper we performed three different methods which provided an extended resolving power for small proteins. Applying the improved techniques, we were able to separate hazelnut proteins into distinct bands below 10 kDa.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(01)00095-0