Phosphorylation of plant actin-depolymerising factor by calmodulin-like domain protein kinase

The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J....

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2001-06, Vol.499 (1), p.97-100
Main Authors: Allwood, Ellen G, Smertenko, Andrei P, Hussey, Patrick J
Format: Article
Language:English
Subjects:
Actin Depolymerizing Factors
Actin-depolymerizing factor
ADF, actin-depolymerising factor
Animals
Blotting, Western
Calmodulin-like domain protein kinase
CDPK, calmodulin-like domain protein kinase
Cofilin
Cytoskeleton
Destrin
Enzyme Inhibitors - pharmacology
Fabaceae - enzymology
Higher plant
Microfilament Proteins - metabolism
Muscle, Skeletal
Phosphorylation
Phosphorylation - drug effects
Phosphoserine - metabolism
Plant Proteins
Plants, Medicinal
Protein Kinase Inhibitors
Protein Kinases - isolation & purification
Protein Kinases - metabolism
Rabbits
W7, N-(6-aminohexyl)-[3H]5-chloro-1-naphthalenesulphonamide
Zea mays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187–193]. We have partially purified the ADF kinase(s) and found it to be calcium-regulated and inhibited by N-(6-aminohexyl)-[ 3H]5-chloro-1-naphthalenesulphonamide. Immunoblotting reveals that calmodulin-like domain protein kinase(s) (CDPK) are enriched in the purified preparation and addition of anti-CDPK to in vitro phosphorylation assays results in the inhibition of ADF phosphorylation. These data strongly suggest that plant ADF is phosphorylated by CDPK(s), a class of protein kinases unique to plants and protozoa.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)02528-5