Practical aspects of liquid chromatography/electrospray tandem mass spectrometry for rapid identification of metabolites of a new antifungal agent SYN-2836 in dog urine

This report presents the structural elucidation of 12 urinary metabolites of SYN‐2836, a new antifungal agent showing extensive metabolism in beagle dogs, using complementary liquid chromatography/tandem mass spectrometry (LC/MS/MS) methodologies. The 12 SYN‐2836 metabolites were readily divided int...

Full description

Saved in:
Bibliographic Details
Published in:Rapid communications in mass spectrometry 2001-07, Vol.15 (13), p.1092-1100
Main Authors: Bu, Hai-Zhi, Poglod, Marzena, Micetich, Ronald G., Khan, Jehangir K.
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - chemistry
Antifungal Agents - pharmacokinetics
Antifungal Agents - urine
Biotransformation
Chromatography, High Pressure Liquid - methods
Dogs
Male
Molecular Structure
Piperazines - chemistry
Piperazines - pharmacokinetics
Piperazines - urine
Spectrometry, Mass, Electrospray Ionization - methods
Triazoles - chemistry
Triazoles - pharmacokinetics
Triazoles - urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This report presents the structural elucidation of 12 urinary metabolites of SYN‐2836, a new antifungal agent showing extensive metabolism in beagle dogs, using complementary liquid chromatography/tandem mass spectrometry (LC/MS/MS) methodologies. The 12 SYN‐2836 metabolites were readily divided into four groups by considering that all three members of each group, although differing in masses, exhibited highly similar product ion mass spectra. This suggests that the metabolites within each group share a common major substructure. Therefore, all the grouped SYN‐2836 metabolites were strategically identified by characterization of the major substructures followed by determination of the additional small substructures. This grouping strategy greatly facilitated the structural elucidation of these metabolites. Other strategies were also employed to achieve as rapid and unambiguous characterization of the SYN‐2836 metabolites as possible. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.347