Inactivated recombinant plant virus protects dogs from a lethal challenge with canine parvovirus

A vaccine based upon a recombinant plant virus (CPMV-PARVO1), displaying a peptide derived from the VP2 capsid protein of canine parvovirus (CPV), has previously been described. To date, studies with the vaccine have utilized viable plant chimaeric particles (CVPs). In this study, CPMV-PARVO1 was in...

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Bibliographic Details
Published in:Vaccine 2001-06, Vol.19 (27), p.3661-3670
Main Authors: Langeveld, Jan P.M, Brennan, Frank R, Martı́nez-Torrecuadrada, Jorge L, Jones, Tim D, Boshuizen, Ronald S, Vela, Carmen, Casal, J.Ignacio, Kamstrup, Søren, Dalsgaard, Kristian, Meloen, Rob H, Bendig, Mary M, Hamilton, William D.O
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bacteriology
Biological and medical sciences
Canine parvovirus
Capsid - therapeutic use
Capsid Proteins
Chimaeric plant virus
Comovirus - immunology
Comovirus - radiation effects
cowpea mosaic virus
Dog Diseases - prevention & control
Dog Diseases - virology
Dogs
Fundamental and applied biological sciences. Psychology
Immunization Schedule
Microbiology
Molecular Sequence Data
Parvoviridae Infections - mortality
Parvoviridae Infections - prevention & control
Parvoviridae Infections - veterinary
Parvovirus, Canine - immunology
Parvovirus, Canine - radiation effects
Recombinant Proteins - therapeutic use
Ultraviolet Rays
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Inactivated - therapeutic use
Vaccines, Synthetic - therapeutic use
Viral Proteins - therapeutic use
Viral Vaccines - therapeutic use
Virology
VP2 protein
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Summary:A vaccine based upon a recombinant plant virus (CPMV-PARVO1), displaying a peptide derived from the VP2 capsid protein of canine parvovirus (CPV), has previously been described. To date, studies with the vaccine have utilized viable plant chimaeric particles (CVPs). In this study, CPMV-PARVO1 was inactivated by UV treatment to remove the possibility of replication of the recombinant plant virus in a plant host after manufacture of the vaccine. We show that the inactivated CVP is able to protect dogs from a lethal challenge with CPV following parenteral immunization with the vaccine. Dogs immunized with the inactivated CPMV-PARVO1 in adjuvant displayed no clinical signs of disease and shedding of CPV in faeces was limited following CPV challenge. All immunized dogs elicited high titres of peptide-specific antibody, which neutralized CPV in vitro. Levels of protection, virus shedding and VP2-specific antibody were comparable to those seen in dogs immunized with the same VP2- peptide coupled to keyhole limpet hemocyanin (KLH). Since plant virus-derived vaccines have the potential for cost-effective manufacture and are not known to replicate in mammalian cells, they represent a viable alternative to current replicating vaccine vectors for development of both human and veterinary vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(01)00083-4