Procalcitonin does not influence the surface expression of inflammatory receptors on whole blood leukocytes

The plasma levels of procalcitonin (PCT) are increased in patients with severe bacterial infections. Its cellular origin and potential pathophysiological function in sepsis is, however, unclear. White blood cells have recently been described to express both PCT mRNA and protein. The aim of this stud...

Full description

Saved in:
Bibliographic Details
Published in:Intensive care medicine 2001-02, Vol.27 (2), p.430-433
Main Authors: JØRGENSEN, P. F, WANG, J. E, SOLBERG, R, THIEMERMANN, C, FOSTER, S. J, AASEN, A. O
Format: Article
Language:English
Subjects:
Analysis of Variance
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Bacterial infections
Biological and medical sciences
Blood
Calcitonin - pharmacology
Calcitonin Gene-Related Peptide
Emergency and intensive care: infection, septic shock
Flow Cytometry
Humans
Infections
Influence
Intensive care medicine
Leukocytes
Leukocytes - metabolism
Medical sciences
Protein Precursors - pharmacology
Receptors, Immunologic - drug effects
Receptors, Immunologic - metabolism
Sepsis
Sepsis - metabolism
Variance analysis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The plasma levels of procalcitonin (PCT) are increased in patients with severe bacterial infections. Its cellular origin and potential pathophysiological function in sepsis is, however, unclear. White blood cells have recently been described to express both PCT mRNA and protein. The aim of this study was to determine whether PCT has any influence on the surface expression of receptors, relevant in inflammation, on human whole blood leukocytes under normal and septic conditions. Venous blood from healthy donors was incubated with PCT (40 ng/ml or 1200 ng/ml) alone or in combination with lipopolysaccharide (LPS, 10 ng/ml) or peptidoglycan (PepG, 10 micrograms/ml) for 6 h. The surface expression of CD14, CD54, CD64, CD80, CD86 and HLA-DR was determined by flow cytometry. We could not detect any influence of PCT on the expression of these receptors. Further studies on potential effects on other cell types during infection seem warranted.
ISSN:0342-4642
1432-1238
DOI:10.1007/s001340000821