A simple regression method for determining the quantity of translated protein in a cell-free protein synthesis system

A simple regression method for quantifying endogenous leucine in S30 extract was developed. This method enabled the quantity of translated protein in a cell-free protein synthesis system to be exactly determined.

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 1999, Vol.88 (3), p.345-347
Main Authors: Kang, Taek Jin, Kang, Sang Hyeon, Jung, Sang Taek, Lee, Eun Yeol, Choi, Cha Yong
Format: Article
Language:English
Subjects:
Amino acids
Biological and medical sciences
Biosynthesis
cell-free protein synthesis system
Diverse techniques
ERITROPOIETINA
ERYTHROPOIETIN
ERYTHROPOIETINE
Extraction
Fundamental and applied biological sciences. Psychology
LEUCINA
LEUCINE
Molecular and cellular biology
PROTEIN SYNTHESIS
regression
Regression analysis
SINTESIS DE PROTEINAS
SYNTHESE PROTEIQUE
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Description
Summary:A simple regression method for quantifying endogenous leucine in S30 extract was developed. This method enabled the quantity of translated protein in a cell-free protein synthesis system to be exactly determined.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(00)80024-9