Effect of repetitive asphyxia on leukocyte-vessel wall interactions in the developing chick intestine

Information on leukocyte-vessel wall interactions (LVWI) during development of the immature intestine is scarce. The authors designed an experimental model for studying the microcirculation in the developing intestine of chick fetuses at days 13 (n = 12), 15 (n = 17), and 17 (n = 19) of incubation (...

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Bibliographic Details
Published in:Journal of pediatric surgery 2000-01, Vol.35 (1), p.49-55
Main Authors: Rouwet, E.V., Beuk, R.J., Heineman, E., Slaaf, D.W., Egbrink, M.G.A. Oude
Format: Article
Language:English
Subjects:
Animals
asphyxia
Asphyxia - embryology
Asphyxia - physiopathology
Biological and medical sciences
Blood Vessels - embryology
Blood Vessels - physiology
Cell Adhesion
Chick Embryo
Development
Diseases of mother, fetus and pregnancy
Gynecology. Andrology. Obstetrics
intestinal microcirculation
Intestines - blood supply
Intestines - embryology
Intestines - pathology
leukocyte-vessel wall interactions
Leukocytes - physiology
Medical sciences
Microcirculation - embryology
Microscopy, Video
Pregnancy. Fetus. Placenta
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Summary:Information on leukocyte-vessel wall interactions (LVWI) during development of the immature intestine is scarce. The authors designed an experimental model for studying the microcirculation in the developing intestine of chick fetuses at days 13 (n = 12), 15 (n = 17), and 17 (n = 19) of incubation (0.6, 0.7, and 0.8 of the incubation time, respectively) using intravital microscopy. The authors investigated whether episodes of asphyxia increase LVWI and induce tissue damage in the developing intestine. Asphyxia was induced by clamping of the chorioallantoic vein for 6 periods of 5 minutes each, with 5-minute intervals, whereas in sham groups a sham procedure was performed. Video recordings were made before as well as 10, 20, and 30 minutes after the end of the asphyxia or sham protocol. Baseline number of rolling leukocytes per minute significantly increased (P
ISSN:0022-3468
1531-5037
DOI:10.1016/S0022-3468(00)80012-3