Changes in Fructose-Induced Production of Glucose in the Rat Liver Following Partial Hepatectomy

The fructose-induced production of glucose in the liver after partial hepatectomy (PH) was evaluated by using the liver-perfusion system. There was no significant difference in plasma glucose level between hepatectomized (HX) and sham-operated (SO) rats at 24 h after surgery, and, thereafter, almost...

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Published in:Archives of biochemistry and biophysics 1999-11, Vol.371 (1), p.53-62
Main Authors: Moriyama, Mitsuaki, Nishisako, Mototaka, Ueda, Junko, Kannan, Yukiko, Ohta, Mitsuaki, Sugano, Tsukasa
Format: Article
Language:English
Subjects:
Acetyl Coenzyme A - metabolism
acetyl-CoA
Animals
Blood Glucose - metabolism
citrate
Citrates - metabolism
Energy Intake
Fatty Acids, Nonesterified - blood
free fatty acids
fructose
Fructose - metabolism
fructose 2,6-bisphosphate
Fructosephosphates - metabolism
Gluconeogenesis
glucose
Glucose - metabolism
Hepatectomy
Kinetics
Lactates - metabolism
Liver - metabolism
liver regeneration
Liver Regeneration - physiology
Male
Phosphoenolpyruvate Carboxykinase (GTP) - metabolism
Pyruvates - metabolism
Rats
Rats, Sprague-Dawley
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Summary:The fructose-induced production of glucose in the liver after partial hepatectomy (PH) was evaluated by using the liver-perfusion system. There was no significant difference in plasma glucose level between hepatectomized (HX) and sham-operated (SO) rats at 24 h after surgery, and, thereafter, almost similar levels were obtained in both groups. However, the level of serum free fatty acids (FFA) was significantly higher in HX rats than that in SO rats at 24 and 48 h after surgery. When both groups of rats were given fructose by gavage, the increment of plasma glucose was significantly larger in HX rats than in SO rats. Lactate infusion failed to increase the rate of glucose production in perfused livers of both HX and SO rats and there was no significant difference in the activity of hepatic phosphoenolpyruvate carboxykinase. By contrast, fructose infusion elicited a large increase in glucose production in the perfused livers of HX rats at 24 and 48 h after PH. The increase was closely associated with not the change in fructose 2,6-bisphosphate levels but the increment of the intracellular levels of citrate. Treatment of octanoate or oleate, which supplies acetyl-CoA via fatty acid oxidation, mimicked the fructose-induced increase in glucose production in SO rats with a concomitant increase in hepatic levels of citrate. These results suggest that the oxidation of FFA may play an important role in glucose production induced by fructose administration during the early phase of liver regeneration.
ISSN:0003-9861
1096-0384
DOI:10.1006/abbi.1999.1422