New P serotype of group A human rotavirus closely related to that of a porcine rotavirus

The VP7 and VP4 genes of two human group A rotavirus strains Mc323 and Mc345 with unique serologic and genomic properties, and isolated in Chiang Mai, Thailand, in 1989 [Urasawa et al. (1992) Journal of Infectious Diseases 166:227–234] were further characterized. The nucleotide and deduced amino aci...

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Bibliographic Details
Published in:Journal of medical virology 2000-01, Vol.60 (1), p.63-69
Main Authors: Okada, Jun-ichi, Urasawa, Tomoko, Kobayashi, Nobumichi, Taniguchi, Koki, Hasegawa, Ayako, Mise, Keiji, Urasawa, Shozo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antigens, Viral
Biological and medical sciences
Capsid - chemistry
Capsid - genetics
Capsid Proteins
Fundamental and applied biological sciences. Psychology
Human rotavirus
Humans
Microbiology
Molecular Sequence Data
Neutralization Tests
nucleotide sequence
P serotype
porcine rotavirus
Rabbits
Reassortant Viruses - genetics
Reassortant Viruses - immunology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Rotavirus
Rotavirus - classification
Rotavirus - genetics
Rotavirus - immunology
Serotyping
Swine
Thailand
Virology
VP4 gene
VP4 genotype
VP4 protein
VP7 gene
VP7 protein
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Summary:The VP7 and VP4 genes of two human group A rotavirus strains Mc323 and Mc345 with unique serologic and genomic properties, and isolated in Chiang Mai, Thailand, in 1989 [Urasawa et al. (1992) Journal of Infectious Diseases 166:227–234] were further characterized. The nucleotide and deduced amino acid sequences of the VP7 genes allowed the classification of both strains as serotype G9. The VP4 genes of both strains are 2,359 nucleotides in length and encode a protein of 775 amino acids like in most human rotaviruses. A comparison of the VP4 amino acid sequence of strain Mc323 with those of strain Mc345 and 24 human and animal rotaviruses representing 20 distinct VP4 genotypes reported to date showed that VP4 of Mc323 and Mc345 belong to genotype 19 previously reported for porcine rotavirus [Burke et al. (1994) Journal of General Virology 75:2205–2212]. To investigate the serological type (P serotype) of these VP4s, six reassortant viruses each containing a distinct VP4 gene characteristic of human rotaviruses and the VP7 gene of porcine rotavirus strain Gottfried (G4) were prepared, and antisera to these reassortants produced in rabbits. In neutralization tests, the P serotype of Mc323 was clearly differentiated from the five major P serotypes reported previously for human rotaviruses, suggesting that Mc323 and Mc345 represent a new human rotavirus P serotype tentatively called P11. J. Med. Virol. 60:63–69, 2000. © 2000 Wiley‐Liss, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/(SICI)1096-9071(200001)60:1<63::AID-JMV11>3.0.CO;2-4