Real-time hybridization chain reaction

In this work, the authors converted into a real-time mode a highly specific method for detection of nucleic acid fragments, which is capable of detecting even single nucleotide substitutions in a target sequence. This method is based on the hybridization chain reaction, which is isothermal, involves...

Full description

Saved in:
Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2008-04, Vol.419 (1), p.53-55
Main Authors: Chemeris, D. A., Nikonorov, Yu. M., Vakhitov, V. A.
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Deoxyribonucleic acid
DNA
DNA - genetics
Fluorescence
Hybridization
Life Sciences
Male
Molecular Biology
Polymerase Chain Reaction - methods
Salmon
Spermatozoa - metabolism
Studies
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, the authors converted into a real-time mode a highly specific method for detection of nucleic acid fragments, which is capable of detecting even single nucleotide substitutions in a target sequence. This method is based on the hybridization chain reaction, which is isothermal, involves no enzymes, and does not involve amplification of nucleic acids. In this reaction, the initiator (target) DNA or RNA molecules (in addition, the latter do not require conversion into DNA) trigger a linear growth of oligonucleotide nanostructures assembled from a pair of hairpins designed in a special manner. A thermocycler equipped with an optical module records in a real-time mode the increasing fluorescence of the corresponding dye due to the damping of quenching effect that takes place at a stepwise elongation of DNA strands and the resulting spatial separation of increasing number of fluorochrome quencher pairs located within individual self-assembling oligonucleotide hairpins.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672908020014