Identification of novel genes associated with conidiation in Beauveria bassiana with suppression subtractive hybridization

The conidiation of the entomopathogenic fungus Beauveria bassiana (Hyphomycete) is a complex process that involves the stage- and cell-type-specific expression of hundreds of genes. The suppression subtractive hybridization method was used to target genes involved in conidiation. Seventeen genes wer...

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Bibliographic Details
Published in:Mycologia 2008, Vol.100 (1), p.20-30
Main Authors: Wu, Jiang, Ridgway, Hayley J., Carpenter, Margaret A., Glare, Travis R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Aspergillus nidulans
Base Sequence
Beauveria
Beauveria - genetics
Beauveria - growth & development
Beauveria - physiology
Beauveria bassiana
Blotting, Northern
Complementary DNA
Conidia
conidiation
DNA, Complementary - genetics
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Fungal - isolation & purification
entomopathogenic fungi
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungi
Gene Expression Regulation, Fungal
Gene Silencing
Genes
Genes, Fungal
Genetic vectors
Genetics/Molecular Biology
Molecular Sequence Data
Mycelium
Mycelium - genetics
Mycelium - growth & development
Mycelium - physiology
Northern blotting
Nucleic Acid Hybridization - methods
nucleotide sequences
Polymerase chain reaction
RNA
sequence analysis
Sequence Analysis, DNA
Spores, Fungal - genetics
Spores, Fungal - growth & development
Spores, Fungal - physiology
SSH
suppression subtractive hybridization
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Summary:The conidiation of the entomopathogenic fungus Beauveria bassiana (Hyphomycete) is a complex process that involves the stage- and cell-type-specific expression of hundreds of genes. The suppression subtractive hybridization method was used to target genes involved in conidiation. Seventeen genes were cloned that potentially were involved in conidia formation. Six of them demonstrated differential expression between conidial and vegetative cultures. Sequence analysis showed three cDNA fragments had similarity to known genes involved in either cellular metabolism or cell regulatory processes. The other cDNA fragments showed low or no similarity to any genes previously described. The full-length cDNA and genomic sequence of a gene designated A43 was isolated. The A43 protein is composed of 180 amino acids and has 34% identity to a RNA-binding region-containing protein. The temporal expression pattern was consistent with the gene being involved in conidiation. The colony morphology of the A43 knock-out mutant had more floccus mycelium than the wild-type and also produced fewer conidia, indicating the A43 gene is involved in B. bassiana conidiation.
ISSN:0027-5514
1557-2536
DOI:10.1080/15572536.2008.11832496