Serum protein profile of rheumatoid arthritis treated with anti-TNF therapy (infliximab)

We analyzed the changes in the serum protein profile by infliximab using two-dimensional gel electrophoresis and mass spectrometry. More than 50 gel spots were seen to increase or decrease in correlation with clinical improvements of RA. The spots corresponding to CRP, C3, and Apo J showed reduced s...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-08, Vol.855 (1), p.66-70
Main Authors: Takeuchi, Tohru, Nakanishi, Toyofumi, Tabushi, Yoko, Hata, Ayu, Shoda, Takeshi, Kotani, Takuya, Shimizu, Akira, Takubo, Takayuki, Makino, Shigeki, Hanafusa, Toshiaki
Format: Article
Language:English
Subjects:
Adolescent
Analysis
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal - therapeutic use
Antirheumatic Agents - therapeutic use
Arthritis, Rheumatoid - blood
Arthritis, Rheumatoid - drug therapy
Biological and medical sciences
Blood Proteins - analysis
Child
Child, Preschool
Chromatography, Affinity
Electrophoresis
Female
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Infant
Infliximab
Male
Matrix assisted laser desorption ionization/time-of-flight mass spectrometry
Medical sciences
Pharmacology. Drug treatments
Rheumatoid arthritis
Tumor necrosis factor
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Summary:We analyzed the changes in the serum protein profile by infliximab using two-dimensional gel electrophoresis and mass spectrometry. More than 50 gel spots were seen to increase or decrease in correlation with clinical improvements of RA. The spots corresponding to CRP, C3, and Apo J showed reduced staining intensity, while the spots corresponding to Apo A-I, RBP, and transthyretin were enhanced. The protein profile of RA patients treated with infliximab was mostly similar to that of normal healthy controls except for several protein spots. This suggested that infliximab normalized the serum protein profile of RA patients, leading to modification in the serum lipid profile and antioxidant status in RA.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2007.02.028