Mouse Model of Erectile Dysfunction Due to Diet-Induced Diabetes Mellitus

Objectives To determine whether diet-induced diabetes mellitus (DM) in mice would reproduce the major features of human erectile dysfunction (ED) because DM is a significant risk factor in the development of ED. Methods In total, 150 C57BL6 (bl6) mice were divided into six groups of 25 mice each. Of...

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Published in:Urology (Ridgewood, N.J.) N.J.), 2007-07, Vol.70 (1), p.196-201
Main Authors: Xie, Donghua, Odronic, Shelley I, Wu, Feihua, Pippen, Anne, Donatucci, Craig F, Annex, Brian H
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Diabetes Mellitus, Experimental - complications
Diabetes. Impaired glucose tolerance
Disease Models, Animal
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Erectile Dysfunction - etiology
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Male
Medical sciences
Mice
Mice, Inbred C57BL
Nephrology. Urinary tract diseases
Urology
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Summary:Objectives To determine whether diet-induced diabetes mellitus (DM) in mice would reproduce the major features of human erectile dysfunction (ED) because DM is a significant risk factor in the development of ED. Methods In total, 150 C57BL6 (bl6) mice were divided into six groups of 25 mice each. Of these 150 mice, 125 were fed a high-fat (45% of total calories) diet for the final 4 (group 2), 8 (group 3), 12 (group 4), 16 (group 5), or 22 (group 6) weeks. Group 1 was fed a normal diet. The mice were 22 to 25 weeks old at study termination. The corporal tissues were harvested and studied for endothelium-dependent and endothelium-independent vasoreactivity, endothelial and smooth muscle cell content by immunohistochemistry, nitric oxide synthase expression by nicotinamide adenine dinucleotide-diaphorase staining, and apoptosis by terminal deoxynucleotidyl transferase biotin- d -UTP nick-end labeling staining. Results The blood glucose levels were greater in groups 2 to 6 compared with those in group 1. The vasoreactivity, endothelial cell content, and smooth muscle/collagen ratio were lower and apoptosis were greater in the DM mice ( P = 0.0001, P = 0.10, P = 0.0002, P
ISSN:0090-4295
1527-9995
DOI:10.1016/j.urology.2007.02.060