Effect of organic mobile phase composition on signal responses for selected polyalkene additive compounds by liquid chromatography–mass spectrometry

The high performance liquid chromatography (HPLC) separation methodology employed in the study of polyalkene additive compounds by atmospheric pressure ionization mass spectrometry (API-MS) was undertaken. Both atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization...

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Bibliographic Details
Published in:Journal of Chromatography A 2008-06, Vol.1193 (1), p.70-78
Main Authors: Duderstadt, Randall E., Fischer, Steven M.
Format: Article
Language:English
Subjects:
Acetone - chemistry
Alkenes - chemistry
Analytical chemistry
Antioxidant
APCI
APPI
Applied sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - methods
Compounding ingredients
Exact sciences and technology
HPLC
Ionization
LC–MS
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
Methanol - chemistry
Mobile phase
Other chromatographic methods
Polymer
Polymer industry, paints, wood
Spectrophotometry, Ultraviolet
Stabilizers (antioxydants, antiozonants, etc.)
Technology of polymers
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Summary:The high performance liquid chromatography (HPLC) separation methodology employed in the study of polyalkene additive compounds by atmospheric pressure ionization mass spectrometry (API-MS) was undertaken. Both atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) were examined. APPI (including dopant-assisted APPI) was found to be an inferior ionization technique to APCI in all cases. APCI ion responses were found to be highly dependent upon the organic solvent type used in the HPLC separations. Namely, employing a water/methanol gradient in place of a water/acetonitrile or a water/acetone gradient yielded improvements in analyte ion intensities between 2.3- and 52-fold for the liquid chromatography–mass spectrometry (LC–MS) experiments. Analyte and mobile phase solvent ionization energies were found to be only partially responsible, whereas mobile phase cluster formation and hydration was also implicated. Mobile phase component modification is demonstrated to be an important consideration when developing new, or modifying existing HPLC separations for use in LC–MS experiments in order to enhance analyte sensitivity for a wide variety of common polyalkene additives.
ISSN:0021-9673
DOI:10.1016/j.chroma.2008.03.086