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Molecular screening and association study of IL15 gene polymorphisms in rheumatoid arthritis
Interleukin 15 (IL-15) is a pleiotropic pro-inflammatory cytokine known to play a relevant role in rheumatoid arthritis (RA) pathogenesis. In this study we aimed to investigate for the first time the contribution of IL15 gene to RA susceptibility. We screened 13 single nucleotide polymorphisms (SNPs...
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Published in: | Cytokine (Philadelphia, Pa.) Pa.), 2007-05, Vol.38 (2), p.84-89 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Interleukin 15 (IL-15) is a pleiotropic pro-inflammatory cytokine known to play a relevant role in rheumatoid arthritis (RA) pathogenesis. In this study we aimed to investigate for the first time the contribution of
IL15 gene to RA susceptibility. We screened 13 single nucleotide polymorphisms (SNPs) localised within
IL15 regulatory regions (promoter, 5′ UTR region and 3′ UTR region) in a total of 420 individuals, who were genotyped by direct sequencing of PCR products. In addition, an association study of these
IL15 SNPs was conducted in three independent case-control cohorts of Spanish Caucasian origin, including a total of 645 RA patients and 656 healthy controls. The presence of the 13 selected
IL15 SNPs in our population was confirmed and no new genetic variants were found. The distribution of the
IL15 selected SNPs in RA patients and controls showed no statistically significant deviation in any of the populations studied. Additionally, we performed a haplotype analysis that revealed three
IL15 haplotype blocks. None of the haplotype blocks was associated with RA susceptibility or severity in the three cohorts analysed. Our results suggest that the
IL15 gene polymorphisms do not appear to play a major role in RA genetic predisposition in our population. |
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ISSN: | 1043-4666 1096-0023 |
DOI: | 10.1016/j.cyto.2007.05.005 |