Bond-rupture immunosensors—A review

It has long been the goal of researchers to develop fast and reliable point-of-care alternatives to existing lab-based tests. A viable point-of-care biosensor is fast, reliable, simple, cost-effective, and detects low concentrations of the target analyte. The target of biosensors is biological such...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2008-07, Vol.23 (12), p.1759-1768
Main Authors: Hirst, Evan R., Yuan, Yong J., Xu, W.L., Bronlund, J.E.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Bond-rupture
Electrochemistry - instrumentation
Equipment Design
Fundamental and applied biological sciences. Psychology
Immunoassay - instrumentation
Immunoassay - methods
Immunosensor
Methods. Procedures. Technologies
QCM
SAW
Technology Assessment, Biomedical
Transducers
Various methods and equipments
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Summary:It has long been the goal of researchers to develop fast and reliable point-of-care alternatives to existing lab-based tests. A viable point-of-care biosensor is fast, reliable, simple, cost-effective, and detects low concentrations of the target analyte. The target of biosensors is biological such as bacteria or virus and as such, the antibody–antigen bond derived from the real immune response is used. Biosensor applications include lab-based tests for the purposes of diagnostics, drug discovery, and research. Additional applications include environmental, food, and agricultural monitoring. The main merits of the bond-rupture method are quick, simple, and capable of discriminating between specific and non-specific interactions. The separation of specific and non-specific bonds is important for working in real-life complex serums such as blood. The bond-rupture technique can provide both qualitative results, the detection of a target, and quantitative results, the concentration of target. Bond-rupture achieves this by a label-free method requiring no pre-processing of the analyte. A piezoelectric transducer such as the quartz crystal microbalance (QCM) shakes the bound particles free from the surface. Other transducers such as Surface Acoustic Wave (SAW) are also considered. The rupture of the bonds is detected as electronic noise. This review article links diverse research areas to build a picture of a field still in development.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2008.02.002