Behavioral and anatomical abnormalities in a sodium iodate-induced model of retinal pigment epithelium degeneration

We characterized changes in the visual behavior of mice in which a loss of the retinal pigment epithelium (RPE) was experimentally induced with intravenous (i.v.) administration of sodium iodate (NaIO 3). We compared and correlated these changes with alterations in neural retinal structure and funct...

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Bibliographic Details
Published in:Experimental eye research 2006-03, Vol.82 (3), p.441-448
Main Authors: Enzmann, Volker, Row, Barry W., Yamauchi, Yasuyuki, Kheirandish, Leila, Gozal, David, Kaplan, Henry J., McCall, Maureen A.
Format: Article
Language:English
Subjects:
Animals
Cued water maze
Dose-Response Relationship, Drug
Histology
Injections, Intravenous
Iodates
Male
Mice
Mice, Inbred C57BL
Models, Animal
Pigment Epithelium of Eye - pathology
Retina - pathology
Retinal Degeneration - pathology
Retinal Degeneration - psychology
RPE
Sodium iodate
Time Factors
Vision, Ocular - drug effects
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Summary:We characterized changes in the visual behavior of mice in which a loss of the retinal pigment epithelium (RPE) was experimentally induced with intravenous (i.v.) administration of sodium iodate (NaIO 3). We compared and correlated these changes with alterations in neural retinal structure and function. RPE loss was induced in 4–6 week old male C57BL/6 mice with an i.v. injection of 1% NaIO 3 at three concentrations: 35, 50, or 70 mg/kg. At 1, 3, 7, 14, 21, and 28 days (d) as well as 6 months post injection (PI) a behavioral test was performed in previously trained mice to evaluate visual function. Eye morphology was then assessed for changes in both the RPE and neural retina. NaIO 3-induced RPE degeneration was both dose and PI time dependent. Our low dose showed no effects, while our high dose caused the most damage, as did longer PI times at our intermediate dose. Using the intermediate dose, no changes were detectable in either visual behavior or retinal morphology at 1 d PI. However, at 3 d PI visual behavior became abnormal and patchy RPE cell loss was observed. From 7 d PI onward, changes in retinal morphology and visual behavior became more severe. At 6 months PI, no recovery was seen in any of these measures in mice administered the intermediate dose. These results show that NaIO 3 dosage and/or time PI can be varied to produce different, yet permanent deficits in retinal morphology and visual function. Thus, this approach should provide a unique system in which the onset and severity of RPE damage, and its consequences can be manipulated. As such, it should be useful in the assessment of rescue or mitigating effects of retinal or stem cell transplantation on visual function.
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2005.08.002