Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis

With the objective of providing inexpensive and reproducible assays for the detection of antibodies indicating exposure to Taenia saginata and Taenia solium, we have evaluated the diagnostic utility of the T. saginata oncosphere adhesion protein (HP6-Tsag), expressed in baculovirus (HP6-Bac) and bac...

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Bibliographic Details
Published in:Parasitology research (1987) 2007-08, Vol.101 (3), p.517-525
Main Authors: Ferrer, Elizabeth, González, Luís Miguel, Martínez-Escribano, José Ángel, González-Barderas, María Eugenia, Cortéz, María Milagros, Dávila, Iris, Harrison, Leslie J. S, Parkhouse, R. Michael E, Gárate, Teresa
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - blood
Antigens, Helminth - genetics
Antigens, Helminth - immunology
Antigens, Helminth - metabolism
Baculoviridae - genetics
Baculoviridae - metabolism
Biological and medical sciences
Blood - parasitology
Cattle - parasitology
Cattle Diseases - diagnosis
Cattle Diseases - parasitology
Cerebrospinal Fluid - parasitology
Cysticercosis - diagnosis
Cysticercosis - parasitology
Cysticercosis - veterinary
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Humans
Invertebrates
Neurocysticercosis - diagnosis
Neurocysticercosis - immunology
Neurocysticercosis - parasitology
Recombinant Proteins - immunology
Swine - parasitology
Swine Diseases - diagnosis
Swine Diseases - parasitology
Taenia saginata - genetics
Taenia saginata - immunology
Taenia solium - immunology
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Summary:With the objective of providing inexpensive and reproducible assays for the detection of antibodies indicating exposure to Taenia saginata and Taenia solium, we have evaluated the diagnostic utility of the T. saginata oncosphere adhesion protein (HP6-Tsag), expressed in baculovirus (HP6-Bac) and bacteria (HP6-GST [glutathione S-transferase]), employing enzyme-linked immunosorbent assays (ELISAs) and sera from T. saginata infected cattle, T. solium infected pigs and serum and cerebrospinal fluid (CSF) samples from clinically defined T. solium neurocysticercosis (NCC) patients. The two recombinant proteins were antigenic in all three systems, with the signal to background ratio of the HP6-Bac ELISA slightly higher than that for the HP6-GST ELISA. Assay performance in cattle was similar to previously described peptide-based ELISA assays, although NCC sample sensitivity/specificity was marginally better. The sensitivity of the HP6-Bac and HP6-GST ELISAs was close for active human NCC (77.4 and 80.6% for serum and 76.9 and 73.1% for CSF samples, respectively). In inactive human NCC, however, the sensitivity of the HP6-Bac ELISA was almost twice that of the HP6-GST ELISA. Because peptides are relatively expensive and recombinant proteins are simple and economical to produce, the latter may provide useful reagents for antibody detection in countries with endemic cysticercosis/NCC.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-007-0507-x