Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator

A micropropagation approach was developed for nine ornamental Prunus species, P. americana, P. cistena, P. glandulosa, P. serrulata 'Kwanzan', P. laurocerasus, P. sargentii, P. tomentosa, P. triloba, P. virginiana 'Schubert', commercially important in North America, and GF305 pea...

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Bibliographic Details
Published in:Plant cell reports 2007-07, Vol.26 (7), p.927-935
Main Authors: Kalinina, Anna, Brown, Daniel C. W
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Infections
Meristem therapy
Methods. Procedures. Technologies
Miscellaneous
P . americana
P. cistena
P. glandulosa
P. laurocerasus
P. sargentii
P. serrulata 'Kwanzan'
P. tomentosa
P. triloba
P. virginiana 'Schubert'
Plant cells and fungal cells
Plant Roots - growth & development
Plant Shoots - growth & development
Plant Viruses - isolation & purification
Prunus
Prunus - growth & development
Prunus - virology
Tissue Culture Techniques
Virus indexing
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Summary:A micropropagation approach was developed for nine ornamental Prunus species, P. americana, P. cistena, P. glandulosa, P. serrulata 'Kwanzan', P. laurocerasus, P. sargentii, P. tomentosa, P. triloba, P. virginiana 'Schubert', commercially important in North America, and GF305 peach, commonly used for Prunus virus indexing. The micropropagation cycle based on proliferation of vegetative tissues includes establishment of tissue culture through introduction of shoot meristems in vitro, shoot proliferation, root induction and plant acclimatization steps and can be completed in 5 months. A meristem sterilization protocol minimized bacterial and fungal contamination. Multiple shoot formation in ornamental Prunus was obtained through the use of 1 mg l-¹ 6-benzyladenine. For GF305 peach, alteration in the sugar composition, fructose instead of sucrose, and addition of 1 mg l-¹ ferulic acid had a significant impact on the shoot proliferation rate and maintenance of long-term in vitro culture. Rooting and plant acclimatization conditions were improved using a two-step protocol with a 4-day root induction in indole-3-butiric acid (IBA)-containing media with consequent 3-week root elongation in IBA-free media. One-month incubation of rooted shoots in a vermiculite-based medium resulted in additional shoot and root growth and provided better acclimatization and plant recovery. The micropropagation approach can be used for maintenance of the clonal properties for Prunus spp. as well as a protocol to support meristem therapy against viral infection.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-007-0315-x