Application of an improved column characterisation system to evaluate the within and between batch variability

The selection of a reversed-phase liquid chromatographic column with suitable selectivity for a particular separation is difficult if the brand name of the column is not known. A project to develop a chromatographic test procedure to characterize reversed-phase liquid chromatography C 18 columns was...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2007-07, Vol.44 (3), p.634-639
Main Authors: Haghedooren, Erik, Kerner, Agnes, Noszál, Béla, Hoogmartens, Jos, Adams, Erwin
Format: Article
Language:English
Subjects:
2,2'-Dipyridyl - analysis
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
C 18 column
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
Reversed-phase liquid chromatography
Test parameters
Time Factors
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Description
Summary:The selection of a reversed-phase liquid chromatographic column with suitable selectivity for a particular separation is difficult if the brand name of the column is not known. A project to develop a chromatographic test procedure to characterize reversed-phase liquid chromatography C 18 columns was started earlier and resulted in a fast, simple, repeatable and reproducible test procedure using four column parameters. Here, this procedure is used to evaluate the diversity of columns originating from the same batch as well as from different batches. The determination of one of the parameters, the retention factor of 2,2′-dipyridyl, was improved and a simplified test procedure is proposed.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2006.08.017