Use of sequence-based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii

Representatives (n = 31) of outbreak strains of Acinetobacter baumannii from five countries fell into three clear groups, designated Groups 1–3, based on their ompA (outer-membrane protein A), csuE (part of a pilus assembly system required for biofilm formation) and blaOXA-51-like (the intrinsic car...

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Bibliographic Details
Published in:Clinical microbiology and infection 2007-08, Vol.13 (8), p.807-815
Main Authors: Turton, J.F., Gabriel, S.N., Valderrey, C., Kaufrnann, M.E., Pitt, T.L.
Format: Article
Language:English
Subjects:
Acinetobacter baumannii
Acinetobacter baumannii - classification
Acinetobacter baumannii - genetics
Acinetobacter baumannii - pathogenicity
Acinetobacter Infections - classification
Acinetobacter Infections - epidemiology
Acinetobacter Infections - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
clonal lineages
Cross Infection - genetics
Cross Infection - microbiology
Disease Outbreaks - classification
Electrophoresis, Gel, Pulsed-Field
Europe - epidemiology
Fundamental and applied biological sciences. Psychology
Humans
Israel - epidemiology
Microbiology
Molecular Sequence Data
multiplex PCR
outbreak strains
PCR
Phylogeny
Polymerase Chain Reaction
Retrospective Studies
sequence-based typing
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Summary:Representatives (n = 31) of outbreak strains of Acinetobacter baumannii from five countries fell into three clear groups, designated Groups 1–3, based on their ompA (outer-membrane protein A), csuE (part of a pilus assembly system required for biofilm formation) and blaOXA-51-like (the intrinsic carbapenemase gene in A. baumannii) gene sequences. With the exception of the closely related alleles within the Group 1 clonal complex, alleles at each locus were highly distinct from each other, with a minimum of 14 nucleotide differences between any two alleles. Isolates within a group shared the same combination of alleles at the three loci, providing compelling evidence that the outbreak strains investigated belonged to three clonal lineages. These corresponded to the previously identified European clones I–III. Sequence differences among the alleles were used to design multiplex PCRs to rapidly assign isolates belonging to particular genotypes to sequence groups. In the UK, genotypes belonging to the Group 1 clonal complex have been particularly successful, accounting for the vast majority of isolates referred from hospitals experiencing problems with Acinetobacter.
ISSN:1198-743X
1469-0691
DOI:10.1111/j.1469-0691.2007.01759.x