Application of polymerase chain reaction fingerprinting to differentiate Ornithobacterium rhinotracheale isolates

Correspondence: 1 Corresponding Author: Kakambi V. Nagaraja, 301 D Vet Science Bldg, 1971 Commonwealth Ave, Saint Paul, MN 55108. nagar001{at}umn.edu Ornithobacterium rhinotracheale (ORT) is an infectious respiratory pathogen of chickens, turkeys, and wild birds. There are 18 serotypes of ORT report...

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Published in:Journal of veterinary diagnostic investigation 2007-07, Vol.19 (4), p.417-420
Main Authors: Thachil, A.J, Velayudhan, B.T, Lopes-Berkas, V.C, Halvorson, D.A, Nagaraja, K.V
Format: Article
Language:English
Subjects:
animal pathogenic bacteria
diagnostic techniques
DNA fingerprinting
DNA Fingerprinting - methods
genetic variation
microbial genetics
new methods
Ornithobacterium - classification
Ornithobacterium - genetics
Ornithobacterium - isolation & purification
Ornithobacterium rhinotracheale
pathogen identification
polymerase chain reaction
Polymerase Chain Reaction - methods
random amplified polymorphic DNA technique
serotypes
Serotyping
strain differences
strains
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Summary:Correspondence: 1 Corresponding Author: Kakambi V. Nagaraja, 301 D Vet Science Bldg, 1971 Commonwealth Ave, Saint Paul, MN 55108. nagar001{at}umn.edu Ornithobacterium rhinotracheale (ORT) is an infectious respiratory pathogen of chickens, turkeys, and wild birds. There are 18 serotypes of ORT reported worldwide. In this study, enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction and random amplified polymorphic DNA assay with Universal M13 primer–based fingerprinting techniques were investigated for their ability to differentiate ORT isolates. The authors examined 50 field isolates and 8 reference strains of ORT for their genetic differences. The fingerprint patterns were compared with serotyping results of ORT by the agar gel precipitation test. M13 fingerprinting revealed different patterns for 6 reference serotypes of ORT that were tested, namely, C, D, E, I, J, and K. Ornithobacterium rhinotracheale reference serotypes A and F yielded indistinguishable fingerprints with M13 fingerprinting. The ERIC 1R technique discerned only 5 of the 8 reference serotypes of ORT. Distinct fingerprints were also found within the ORT serotypes with both techniques. From 58 isolates of ORT that were fingerprinted belonging to 8 ORT serotypes, 10 different fingerprints were obtained with M13 fingerprinting and 6 different fingerprints were obtained with ERIC 1R fingerprinting. M13 fingerprinting technique was found to be more discriminative in differentiating ORT isolates than the ERIC 1R fingerprinting technique. These results suggest that fingerprinting techniques may be a more discerning tool for characterizing ORT isolates than the serological test using the agar gel precipitation test. This fingerprinting technique could potentially be a valuable tool in identifying an isolate from a clinical outbreak of ORT infection for development of an autogenous vaccine. Key Words: Ornithobacterium rhinotracheale • PCR fingerprinting • subtyping
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870701900415