Acquisition of CD80 (B7-1) by T Cells

Activation of T cells usually requires two signals. Signal 1 is mediated via a peptide-MHC on the APC; signal 2 is mediated via a costimulatory molecule on the APC surface. We demonstrate here that naive CD4(+) T cells actually acquire the costimulatory molecule CD80 (B7-1) from syngeneic APCs after...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2001-02, Vol.166 (4), p.2505-2513
Main Authors: Sabzevari, Helen, Kantor, Judy, Jaigirdar, Adnan, Tagaya, Yutaka, Naramura, Mayumi, Hodge, James W, Bernon, John, Schlom, Jeffrey
Format: Article
Language:English
Subjects:
AB7-1 antigen
Animals
Antigen Presentation - genetics
Antigen-Presenting Cells - immunology
Antigen-Presenting Cells - metabolism
Apoptosis - genetics
Apoptosis - immunology
B7-1 Antigen - biosynthesis
B7-1 Antigen - genetics
B7-1 Antigen - metabolism
CD28 Antigens - genetics
CD28 Antigens - metabolism
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - metabolism
CD80 antigen
Cell Line
Cells, Cultured
COS Cells
Cycloheximide - pharmacology
Dose-Response Relationship, Immunologic
Immunologic Memory - genetics
Interphase - genetics
Interphase - immunology
Lymphocyte Activation - drug effects
Lymphocyte Activation - genetics
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Polymerase Chain Reaction
Protein Binding - genetics
Protein Binding - immunology
Protein Synthesis Inhibitors - pharmacology
RNA, Messenger - analysis
Signal Transduction - genetics
Signal Transduction - immunology
Species Specificity
T-Lymphocyte Subsets - drug effects
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - metabolism
Tumor Cells, Cultured
Up-Regulation - drug effects
Up-Regulation - genetics
Up-Regulation - immunology
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Summary:Activation of T cells usually requires two signals. Signal 1 is mediated via a peptide-MHC on the APC; signal 2 is mediated via a costimulatory molecule on the APC surface. We demonstrate here that naive CD4(+) T cells actually acquire the costimulatory molecule CD80 (B7-1) from syngeneic APCs after activation. This phenomenon was demonstrated showing acquisition of CD80 by T cells from CD80/CD86 (B7-2) knockout mice, and by treating T cells with cyclohexamide to further rule out endogenous expression of CD80 by T cells. Moreover, no CD80 mRNA could be detected in T cells that had acquired CD80. The amount of acquisition of CD80 by T cells was shown to be directly related to both the strength of signal 1 and the amount of CD80 on the APC. Specificity of this acquisition was also shown by the lack of acquisition by T cells from CD28 knockout mice (implicating CD28 in this process), the lack of acquisition of CD40 (another molecule on the APC surface) by T cells, and confocal microscopy studies. We demonstrate for the first time that 1) naive T cells, following acquisition of CD80 from APCs, were themselves shown to be capable of acting as APCs; and 2) memory T cells that have acquired CD80 from APCs undergo apoptosis in the presence of increased levels of signal 1. Thus we demonstrate both immunostimulatory and immunoregulatory functions as a result of CD80 acquisition by different T cell populations.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.166.4.2505