Innate resistance to flavivirus infection in mice controlled by Flv is nitric oxide-independent

Department of Microbiology, University of Western Australia, Nedlands, WA 6907, Australia 1 Author for correspondence: Nadezda Urosevic. Fax +61 8 9346 2912. e-mail nadia{at}cyllene.uwa.edu.au Innate resistance to flaviviruses in mice is active in the brain where it restricts virus replication. This...

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Bibliographic Details
Published in:Journal of general virology 2001-03, Vol.82 (3), p.603-607
Main Authors: Silvia, Ondine J, Shellam, Geoffrey R, Urosevic, Nadezda
Format: Article
Language:English
Subjects:
Animals
Brain - virology
Cells, Cultured
chromosome 5
Encephalitis Virus, Murray Valley - growth & development
Encephalitis Virus, Murray Valley - immunology
Encephalitis Virus, Murray Valley - physiology
Encephalitis, Arbovirus - immunology
Encephalitis, Arbovirus - metabolism
Flavivirus
Flavivirus - growth & development
Flavivirus - immunology
Flavivirus - physiology
Flavivirus Infections - immunology
Flavivirus Infections - metabolism
Flavivirus Infections - virology
Flv gene
Immunity, Innate - genetics
Lipopolysaccharides - pharmacology
Macrophages, Peritoneal - cytology
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - virology
Mice
Mice, Inbred C3H
Nitric Oxide - metabolism
Nos1 gene
Virus Replication - drug effects
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Summary:Department of Microbiology, University of Western Australia, Nedlands, WA 6907, Australia 1 Author for correspondence: Nadezda Urosevic. Fax +61 8 9346 2912. e-mail nadia{at}cyllene.uwa.edu.au Innate resistance to flaviviruses in mice is active in the brain where it restricts virus replication. This resistance is controlled by a single genetic locus, Flv , located on mouse chromosome 5 near the locus encoding the neuronal form of nitric oxide synthase ( Nos1 ). Since nitric oxide (NO) has been implicated in antiviral activity, its involvement in natural resistance to flaviviruses has been hypothesized. Here we present data on NO production before and during flavivirus infection in both brain tissue and peritoneal macrophages from two flavivirus-resistant ( Flv r ) and one congenic susceptible ( Flv s ) mouse strains. This study provides evidence that NO is not involved in the expression of flavivirus resistance controlled by Flv since: ( a ) there is no difference in brain tissue NO levels between susceptible and resistant mice, and ( b ) lipopolysaccharide-induced NO does not abrogate the difference in flavivirus replication in peritoneal macrophages from susceptible and resistant mice.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-82-3-603