Expression of TMEFF1 mRNA in the mouse central nervous system: precise examination and comparative studies of TMEFF1 and TMEFF2

TMEFF1 and TMEFF2 are putative transmembrane proteins comprised of one epidermal growth factor (EGF)-like domain and two follistatin-like domains. Both TMEFF1 and TMEFF2 are predominantly expressed in the brain. We previously demonstrated that recombinant TMEFF2 protein can promote survival of neuro...

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Published in:Brain research. Molecular brain research. 2001-01, Vol.86 (1-2), p.48-55
Main Authors: Kanemoto, Naohide, Horie, Masato, Omori, Kuniko, Nishino, Naoki, Kondo, Mari, Noguchi, Koichi, Tanigami, Akira
Format: Article
Language:English
Subjects:
Animals
Biochemistry and metabolism
Biological and medical sciences
Blotting, Northern
Brain Chemistry - genetics
Central nervous system
follistatin
Fundamental and applied biological sciences. Psychology
Gene Expression - physiology
In Situ Hybridization
Male
Membrane Proteins - genetics
Mice
Mice, Inbred C57BL
Mouse
RNA, Messenger - analysis
Survival factor
TMEFF protein
TMEFF1
TMEFF2
Vertebrates: nervous system and sense organs
Xenopus Proteins
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Summary:TMEFF1 and TMEFF2 are putative transmembrane proteins comprised of one epidermal growth factor (EGF)-like domain and two follistatin-like domains. Both TMEFF1 and TMEFF2 are predominantly expressed in the brain. We previously demonstrated that recombinant TMEFF2 protein can promote survival of neurons in primary culture and determined expression sites of TMEFF2 mRNA in the mouse central nervous system. To extend our understanding of TMEFF protein functions, we compared precise sites of expression of TMEFF1 and TMEFF2 mRNA using in situ hybridization analysis. Although both TMEFF genes are widely expressed in the brain, they exhibit different patterns of expression. TMEFF1 showed comparatively higher signals in the pyramidal cells of fifth layer of the cerebral neocortex, CA3, CA1 and subiculum regions of the hippocampus, locus coeruleus, and dentate cerebellar nucleus. In contrast, TMEFF2 is highly expressed in the medial habenular, CA2, CA3 and dentate gyrus region of the hippocampus, corpus callosum, cerebellar cortex and cranial nerve nuclei (III, IV, VII, X, XII). The results presented here indicate that expression of TMEFF1 and TMEFF2 are regulated differently and that they play region-specific roles in the central nervous system.
ISSN:0169-328X
1872-6941
DOI:10.1016/S0169-328X(00)00257-6