Comparative evaluation of the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays and the AMPLICOR HIV-1 MONITOR v1.5 test for the quantitation of human immunodeficiency virus type 1 RNA in plasma

HIV-1 RNA measurements from 84 plasma specimens obtained with the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays (Chiron Diagnostics, Emeryville, CA) and with the AMPLICOR HIV-1 MONITOR™ Test, version 1.5 with ultra-sensitive specimen preparation (Roche Diagnostic Systems, Inc., Branchburg, NJ) were...

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Bibliographic Details
Published in:Journal of virological methods 2001, Vol.91 (1), p.67-74
Main Authors: Anastassopoulou, Cleo G, Touloumi, Giota, Katsoulidou, Antigoni, Hatzitheodorou, Helen, Pappa, Maroudia, Paraskevis, Dimitrios, Lazanas, Marios, Gargalianos, Panagiotis, Hatzakis, Angelos
Format: Article
Language:English
Subjects:
Adult
Amplicor
Biological and medical sciences
Branched DNA
Branched DNA Signal Amplification Assay
DNA Probes
Female
Fundamental and applied biological sciences. Psychology
HIV Infections - virology
HIV-1 - genetics
HIV-1 - isolation & purification
HIV-1 RNA
Human immunodeficiency virus 1
Humans
Male
Microbiology
Quantiplex assay
Reagent Kits, Diagnostic
Reverse Transcriptase Polymerase Chain Reaction
RNA levels
RNA, Viral - blood
Viral Load
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Summary:HIV-1 RNA measurements from 84 plasma specimens obtained with the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays (Chiron Diagnostics, Emeryville, CA) and with the AMPLICOR HIV-1 MONITOR™ Test, version 1.5 with ultra-sensitive specimen preparation (Roche Diagnostic Systems, Inc., Branchburg, NJ) were compared. The absolute RNA values of tested specimens differed significantly between bDNA 2.0 and bDNA 3.0 or Monitor v1.5 measurements (Wilcoxon signed-rank test P
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(00)00245-7