Phage display of peptide / major histocompatibility class I complexes

Major histocompatibility complex class I (MHC‐I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC‐I for peptides a...

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Bibliographic Details
Published in:European journal of immunology 2001-01, Vol.31 (1), p.32-38
Main Authors: Vest Hansen, Nils Jakob, Østergaard Pedersen, Lars, Stryhn, Anette, Buus, Søren
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteriophages - genetics
Base Sequence
Histocompatibility Antigens Class I - chemistry
Histocompatibility Antigens Class I - metabolism
MHC class I
Molecular Sequence Data
Peptides - metabolism
Phage display
Protein Folding
Receptors, Antigen, T-Cell - metabolism
Recombinant Fusion Proteins - metabolism
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Summary:Major histocompatibility complex class I (MHC‐I) molecules sample peptides from the intracellular environment and present them to cytotoxic T cells (CTL). To establish a selection system, and, thereby, enable a library approach to identify the specificities involved (that of the MHC‐I for peptides and subsequently that ot the T cell receptor for peptide‐MHC‐I complex), we have fused a single chain peptide‐MHC‐I complex to the phage minor coat protein, gpIII, and displayed it on filamentous phage. Expression of peptide‐MHC‐I complexes was shown with relevant conformation‐specific monoclonal antibodies and, more importantly, with a unique "T cell receptor‐like" (i. e. peptide‐specific, MHC‐I‐restricted) antibody. Thus, properly assembled and folded peptide‐MHC‐I complexes can be displayed on filamentous phage. Despite the successful display, interaction with T cells could not be demonstrated.
ISSN:0014-2980
1521-4141
DOI:10.1002/1521-4141(200101)31:1<32::AID-IMMU32>3.0.CO;2-6