Transcriptional coactivator, CIITA, is an acetyltransferase that bypasses a promoter requirement for TAF(II)250

The CIITA coactivator is essential for transcriptional activation of MHC class II genes and mediates enhanced MHC class I transcription. We now report that CIITA contains an intrinsic acetyltransferase (AT) activity that maps to a region within the N-terminal segment of CIITA, between amino acids 94...

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Bibliographic Details
Published in:Molecular cell 2001-01, Vol.7 (1), p.105-115
Main Authors: Raval, A, Howcroft, T K, Weissman, J D, Kirshner, S, Zhu, X S, Yokoyama, K, Ting, J, Singer, D S
Format: Article
Language:English
Subjects:
Acetyltransferases - genetics
Acetyltransferases - metabolism
Animals
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Gene Expression Regulation
GTP-Binding Proteins - chemistry
GTP-Binding Proteins - genetics
GTP-Binding Proteins - metabolism
HeLa Cells
Histocompatibility Antigens Class I - genetics
Histocompatibility Antigens Class II - genetics
Humans
Nuclear Proteins
Promoter Regions, Genetic - physiology
Protein Structure, Tertiary
Trans-Activators - chemistry
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptional Activation - physiology
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Summary:The CIITA coactivator is essential for transcriptional activation of MHC class II genes and mediates enhanced MHC class I transcription. We now report that CIITA contains an intrinsic acetyltransferase (AT) activity that maps to a region within the N-terminal segment of CIITA, between amino acids 94 and 132. The AT activity is regulated by the C-terminal GTP-binding domain and is stimulated by GTP. CIITA-mediated transactivation depends on the AT activity. Further, we report that, although constitutive MHC class I transcription depends on TAF(II)250, CIITA activates the promoter in the absence of functional TAF(II)250.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(01)00159-9