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Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women
A cysteine proteinase of 30kDa (CP30) of Trichomonas vaginalis, is known to play a role in cytoadherence of the parasite to host cells. However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fres...
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| Published in: | Experimental parasitology 2007-08, Vol.116 (4), p.399-406 |
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| cites | cdi_FETCH-LOGICAL-c424t-251355ec40713e7349f8d3819e54d0a00ba1c353e7151db8a79b694fd43b25883 |
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| container_start_page | 399 |
| container_title | Experimental parasitology |
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| creator | Yadav, Manisha Dubey, M.L. Gupta, Indu Bhatti, Gurjeet Malla, Nancy |
| description | A cysteine proteinase of 30kDa (CP30) of Trichomonas vaginalis, is known to play a role in cytoadherence of the parasite to host cells. However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fresh and long-term culture maintained T. vaginalis isolates each from symptomatic and asymptomatic women by substrate gel electrophoresis and immunoblotting. Though CP30 was detected in all the fresh isolates from 20 symptomatic and 20 asymptomatic women, the intensity of CP30 band was significantly higher in isolates from symptomatic as compared to asymptomatic women indicating higher expression in former. CP30 was found in all the 20 long-term cultured isolates from symptomatic whereas only in 70% of asymptomatic women indicating that CP30 expression is a more stable characteristic of symptomatic isolates. The isolates from symptomatic women, demonstrated significantly higher cytoadherence to VECs as compared to asymptomatic women. In both the types of isolates, this cytoadherence was inhibited significantly by CP30 specific hyperimmune serum. These results confirm that CP30 is an important virulence factor of T. vaginalis and has an important role in cytoadherence to VECs and thus has a role in pathogenesis of trichomoniasis. |
| doi_str_mv | 10.1016/j.exppara.2007.02.007 |
| format | article |
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However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fresh and long-term culture maintained T. vaginalis isolates each from symptomatic and asymptomatic women by substrate gel electrophoresis and immunoblotting. Though CP30 was detected in all the fresh isolates from 20 symptomatic and 20 asymptomatic women, the intensity of CP30 band was significantly higher in isolates from symptomatic as compared to asymptomatic women indicating higher expression in former. CP30 was found in all the 20 long-term cultured isolates from symptomatic whereas only in 70% of asymptomatic women indicating that CP30 expression is a more stable characteristic of symptomatic isolates. The isolates from symptomatic women, demonstrated significantly higher cytoadherence to VECs as compared to asymptomatic women. In both the types of isolates, this cytoadherence was inhibited significantly by CP30 specific hyperimmune serum. These results confirm that CP30 is an important virulence factor of T. vaginalis and has an important role in cytoadherence to VECs and thus has a role in pathogenesis of trichomoniasis.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2007.02.007</identifier><identifier>PMID: 17420015</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; c.p.m ; Cell Adhesion - immunology ; Cell Adhesion - physiology ; count per minute ; CP30 ; Cysteine Endopeptidases - immunology ; Cysteine Endopeptidases - metabolism ; cysteine proteinase 30 ; Cytoadherence ; E-64 ; Electrophoresis, Polyacrylamide Gel ; Epithelial Cells - parasitology ; Female ; Fundamental and applied biological sciences. Psychology ; HIS ; Humans ; hyperimmune serum ; Immune Sera - immunology ; l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane ; Life cycle. Host-agent relationship. Pathogenesis ; normal rabbit serum ; NRS ; Protozoa ; Trichomonas vaginalis ; Trichomonas vaginalis - enzymology ; Trichomonas vaginalis - immunology ; Trichomonas vaginalis - isolation & purification ; Trichomonas vaginalis - pathogenicity ; Trichomonas Vaginitis - parasitology ; Trichomoniasis ; Urine - parasitology ; Vagina - cytology ; Vagina - parasitology ; vaginal epithelial cells ; VECs ; Virulence Factors - immunology ; Virulence Factors - metabolism</subject><ispartof>Experimental parasitology, 2007-08, Vol.116 (4), p.399-406</ispartof><rights>2007 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-251355ec40713e7349f8d3819e54d0a00ba1c353e7151db8a79b694fd43b25883</citedby><cites>FETCH-LOGICAL-c424t-251355ec40713e7349f8d3819e54d0a00ba1c353e7151db8a79b694fd43b25883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18829772$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17420015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yadav, Manisha</creatorcontrib><creatorcontrib>Dubey, M.L.</creatorcontrib><creatorcontrib>Gupta, Indu</creatorcontrib><creatorcontrib>Bhatti, Gurjeet</creatorcontrib><creatorcontrib>Malla, Nancy</creatorcontrib><title>Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>A cysteine proteinase of 30kDa (CP30) of Trichomonas vaginalis, is known to play a role in cytoadherence of the parasite to host cells. However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fresh and long-term culture maintained T. vaginalis isolates each from symptomatic and asymptomatic women by substrate gel electrophoresis and immunoblotting. Though CP30 was detected in all the fresh isolates from 20 symptomatic and 20 asymptomatic women, the intensity of CP30 band was significantly higher in isolates from symptomatic as compared to asymptomatic women indicating higher expression in former. CP30 was found in all the 20 long-term cultured isolates from symptomatic whereas only in 70% of asymptomatic women indicating that CP30 expression is a more stable characteristic of symptomatic isolates. The isolates from symptomatic women, demonstrated significantly higher cytoadherence to VECs as compared to asymptomatic women. In both the types of isolates, this cytoadherence was inhibited significantly by CP30 specific hyperimmune serum. These results confirm that CP30 is an important virulence factor of T. vaginalis and has an important role in cytoadherence to VECs and thus has a role in pathogenesis of trichomoniasis.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>c.p.m</subject><subject>Cell Adhesion - immunology</subject><subject>Cell Adhesion - physiology</subject><subject>count per minute</subject><subject>CP30</subject><subject>Cysteine Endopeptidases - immunology</subject><subject>Cysteine Endopeptidases - metabolism</subject><subject>cysteine proteinase 30</subject><subject>Cytoadherence</subject><subject>E-64</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Epithelial Cells - parasitology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIS</subject><subject>Humans</subject><subject>hyperimmune serum</subject><subject>Immune Sera - immunology</subject><subject>l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>normal rabbit serum</subject><subject>NRS</subject><subject>Protozoa</subject><subject>Trichomonas vaginalis</subject><subject>Trichomonas vaginalis - enzymology</subject><subject>Trichomonas vaginalis - immunology</subject><subject>Trichomonas vaginalis - isolation & purification</subject><subject>Trichomonas vaginalis - pathogenicity</subject><subject>Trichomonas Vaginitis - parasitology</subject><subject>Trichomoniasis</subject><subject>Urine - parasitology</subject><subject>Vagina - cytology</subject><subject>Vagina - parasitology</subject><subject>vaginal epithelial cells</subject><subject>VECs</subject><subject>Virulence Factors - immunology</subject><subject>Virulence Factors - metabolism</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkcGP1CAYxYnRuLOrf4KGi95aPyi0cDJmoq7JJl7WM6Hw1TBpaYXOuvPfy2SarLc9PQK_Bx_vEfKOQc2AtZ8ONT4ui0225gBdDbwu8oLsGGiouBD6JdkBMFEJpcUVuc75AACKcfGaXLFOFBeTOzLuT3nFEJEuaT4vbEbaAA2RujHE4OxIQ55Hu2Km80Dva_pgfxdsDJkOaZ5oPk3LOk92DY7a6Kn9fyPEAd2Knv6dJ4xvyKvBjhnfbnpDfn37er-_re5-fv-x_3JXOcHFWnHJGinRCehYg10j9KB8o5hGKTxYgN4y18hyxCTzvbKd7lstBi-ankulmhvy8XJv-dOfI-bVTCE7HEcbcT5m00ELSjP-LMhLYG0rdAHlBXRpzjnhYJYUJptOhoE592EOZuvDnPswwE2R4nu_PXDsJ_RPrq2AAnzYAJtL1kOy0YX8xCnFddedJ_184bDk9hAwmewCRoc-pJKw8XN4ZpR_Scqrkg</recordid><startdate>20070801</startdate><enddate>20070801</enddate><creator>Yadav, Manisha</creator><creator>Dubey, M.L.</creator><creator>Gupta, Indu</creator><creator>Bhatti, Gurjeet</creator><creator>Malla, Nancy</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20070801</creationdate><title>Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women</title><author>Yadav, Manisha ; Dubey, M.L. ; Gupta, Indu ; Bhatti, Gurjeet ; Malla, Nancy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-251355ec40713e7349f8d3819e54d0a00ba1c353e7151db8a79b694fd43b25883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>c.p.m</topic><topic>Cell Adhesion - immunology</topic><topic>Cell Adhesion - physiology</topic><topic>count per minute</topic><topic>CP30</topic><topic>Cysteine Endopeptidases - immunology</topic><topic>Cysteine Endopeptidases - metabolism</topic><topic>cysteine proteinase 30</topic><topic>Cytoadherence</topic><topic>E-64</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Epithelial Cells - parasitology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIS</topic><topic>Humans</topic><topic>hyperimmune serum</topic><topic>Immune Sera - immunology</topic><topic>l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>normal rabbit serum</topic><topic>NRS</topic><topic>Protozoa</topic><topic>Trichomonas vaginalis</topic><topic>Trichomonas vaginalis - enzymology</topic><topic>Trichomonas vaginalis - immunology</topic><topic>Trichomonas vaginalis - isolation & purification</topic><topic>Trichomonas vaginalis - pathogenicity</topic><topic>Trichomonas Vaginitis - parasitology</topic><topic>Trichomoniasis</topic><topic>Urine - parasitology</topic><topic>Vagina - cytology</topic><topic>Vagina - parasitology</topic><topic>vaginal epithelial cells</topic><topic>VECs</topic><topic>Virulence Factors - immunology</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yadav, Manisha</creatorcontrib><creatorcontrib>Dubey, M.L.</creatorcontrib><creatorcontrib>Gupta, Indu</creatorcontrib><creatorcontrib>Bhatti, Gurjeet</creatorcontrib><creatorcontrib>Malla, Nancy</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yadav, Manisha</au><au>Dubey, M.L.</au><au>Gupta, Indu</au><au>Bhatti, Gurjeet</au><au>Malla, Nancy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2007-08-01</date><risdate>2007</risdate><volume>116</volume><issue>4</issue><spage>399</spage><epage>406</epage><pages>399-406</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>A cysteine proteinase of 30kDa (CP30) of Trichomonas vaginalis, is known to play a role in cytoadherence of the parasite to host cells. However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fresh and long-term culture maintained T. vaginalis isolates each from symptomatic and asymptomatic women by substrate gel electrophoresis and immunoblotting. Though CP30 was detected in all the fresh isolates from 20 symptomatic and 20 asymptomatic women, the intensity of CP30 band was significantly higher in isolates from symptomatic as compared to asymptomatic women indicating higher expression in former. CP30 was found in all the 20 long-term cultured isolates from symptomatic whereas only in 70% of asymptomatic women indicating that CP30 expression is a more stable characteristic of symptomatic isolates. The isolates from symptomatic women, demonstrated significantly higher cytoadherence to VECs as compared to asymptomatic women. In both the types of isolates, this cytoadherence was inhibited significantly by CP30 specific hyperimmune serum. These results confirm that CP30 is an important virulence factor of T. vaginalis and has an important role in cytoadherence to VECs and thus has a role in pathogenesis of trichomoniasis.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>17420015</pmid><doi>10.1016/j.exppara.2007.02.007</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0014-4894 |
| ispartof | Experimental parasitology, 2007-08, Vol.116 (4), p.399-406 |
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| subjects | Animals Biological and medical sciences c.p.m Cell Adhesion - immunology Cell Adhesion - physiology count per minute CP30 Cysteine Endopeptidases - immunology Cysteine Endopeptidases - metabolism cysteine proteinase 30 Cytoadherence E-64 Electrophoresis, Polyacrylamide Gel Epithelial Cells - parasitology Female Fundamental and applied biological sciences. Psychology HIS Humans hyperimmune serum Immune Sera - immunology l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane Life cycle. Host-agent relationship. Pathogenesis normal rabbit serum NRS Protozoa Trichomonas vaginalis Trichomonas vaginalis - enzymology Trichomonas vaginalis - immunology Trichomonas vaginalis - isolation & purification Trichomonas vaginalis - pathogenicity Trichomonas Vaginitis - parasitology Trichomoniasis Urine - parasitology Vagina - cytology Vagina - parasitology vaginal epithelial cells VECs Virulence Factors - immunology Virulence Factors - metabolism |
| title | Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women |
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