Cysteine proteinase 30 in clinical isolates of T. vaginalis from symptomatic and asymptomatic infected women

A cysteine proteinase of 30kDa (CP30) of Trichomonas vaginalis, is known to play a role in cytoadherence of the parasite to host cells. However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fres...

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Bibliographic Details
Published in:Experimental parasitology 2007-08, Vol.116 (4), p.399-406
Main Authors: Yadav, Manisha, Dubey, M.L., Gupta, Indu, Bhatti, Gurjeet, Malla, Nancy
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
c.p.m
Cell Adhesion - immunology
Cell Adhesion - physiology
count per minute
CP30
Cysteine Endopeptidases - immunology
Cysteine Endopeptidases - metabolism
cysteine proteinase 30
Cytoadherence
E-64
Electrophoresis, Polyacrylamide Gel
Epithelial Cells - parasitology
Female
Fundamental and applied biological sciences. Psychology
HIS
Humans
hyperimmune serum
Immune Sera - immunology
l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane
Life cycle. Host-agent relationship. Pathogenesis
normal rabbit serum
NRS
Protozoa
Trichomonas vaginalis
Trichomonas vaginalis - enzymology
Trichomonas vaginalis - immunology
Trichomonas vaginalis - isolation & purification
Trichomonas vaginalis - pathogenicity
Trichomonas Vaginitis - parasitology
Trichomoniasis
Urine - parasitology
Vagina - cytology
Vagina - parasitology
vaginal epithelial cells
VECs
Virulence Factors - immunology
Virulence Factors - metabolism
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Summary:A cysteine proteinase of 30kDa (CP30) of Trichomonas vaginalis, is known to play a role in cytoadherence of the parasite to host cells. However, the CP30 activity in clinical isolates from symptomatic and asymptomatic patients has not been analyzed. In the present study, CP30 was detected in 20 fresh and long-term culture maintained T. vaginalis isolates each from symptomatic and asymptomatic women by substrate gel electrophoresis and immunoblotting. Though CP30 was detected in all the fresh isolates from 20 symptomatic and 20 asymptomatic women, the intensity of CP30 band was significantly higher in isolates from symptomatic as compared to asymptomatic women indicating higher expression in former. CP30 was found in all the 20 long-term cultured isolates from symptomatic whereas only in 70% of asymptomatic women indicating that CP30 expression is a more stable characteristic of symptomatic isolates. The isolates from symptomatic women, demonstrated significantly higher cytoadherence to VECs as compared to asymptomatic women. In both the types of isolates, this cytoadherence was inhibited significantly by CP30 specific hyperimmune serum. These results confirm that CP30 is an important virulence factor of T. vaginalis and has an important role in cytoadherence to VECs and thus has a role in pathogenesis of trichomoniasis.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2007.02.007