Evaluation of Fatty Acid Extraction Methods for Thraustochytrium sp. ONC-T18

Various extraction methods were assessed in their capacity to extract fatty acids from a dried biomass of Thraustochytrium sp. ONC-T18. Direct saponification using KOH in ethanol or in hexane:ethanol was one of the most efficient techniques to extract lipids (697 mg g-1). The highest amount of fatty...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-06, Vol.55 (12), p.4795-4801
Main Authors: Burja, Adam M, Armenta, Roberto E, Radianingtyas, Helia, Barrow, Colin J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biomass
Cell Wall - ultrastructure
docosahexaenoic acid
dried biosolids
Esters
extraction
fatty acids
Fatty Acids - chemistry
Fatty Acids - isolation & purification
Fermentation
Food engineering
Food industries
Fundamental and applied biological sciences. Psychology
Fungi - chemistry
Fungi - growth & development
Fungi - ultrastructure
General aspects
Labyrinthulomycetes
Lipids - chemistry
Lipids - isolation & purification
myristic acid
palmitic acid
palmitoleic acid
saponification
Solvents
Thraustochytrium
transesterification
ultrasonics
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Summary:Various extraction methods were assessed in their capacity to extract fatty acids from a dried biomass of Thraustochytrium sp. ONC-T18. Direct saponification using KOH in ethanol or in hexane:ethanol was one of the most efficient techniques to extract lipids (697 mg g-1). The highest amount of fatty acids (714 mg g-1) was extracted using a miniaturized Bligh and Dyer extraction technique. The use of ultrasonics to break down cell walls while extracting with solvents (methanol:chloroform) also offered high extraction yields of fatty acids (609 mg g-1). Moreover, when the transesterification mixture used for a direct transesterification method was doubled, the extraction of fatty acids increased approximately 77% (from 392 to 696 mg g-1). This work showed that Thraustochytrium sp. ONC-T18 has the ability to produce over 700 mg g-1 of lipids, including more than 165 mg g-1 of docosahexaenoic acid, which makes this microorganism a potential candidate for the commercial production of polyunsaturated fatty acids. Finally, other lipids, such as myristic, palmitic, palmitoleic, and oleic acids, were also produced and recovered in significant amounts (54, 196, 123, and 81 mg g-1), respectively. Keywords: Docosahexaenoic acid; eicosapentaenoic acid; polyunsaturated fatty acids; ω-3 fatty acids; Thraustochytrium sp. ONC-T18, lipids; extractions; ultrasonics
ISSN:0021-8561
1520-5118
DOI:10.1021/jf070412s