Analysis of lignans from Phyllanthus niruri L. in plasma using a simple HPLC method with fluorescence detection and its application in a pharmacokinetic study

A simple analytical method using HPLC with fluorescence detection was developed for the simultaneous determination of four lignans, phyllanthin ( 1), hypophyllanthin ( 2), phyltetralin ( 3) and niranthin ( 4) from Phyllanthus niruri L. in plasma. The method recorded limits of detection for 1, 2, 3 a...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-06, Vol.852 (1), p.138-144
Main Authors: Murugaiyah, Vikneswaran, Chan, Kit Lam
Format: Article
Language:English
Subjects:
Absolute oral bioavailability
Analysis
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Euphorbiaceae
Fundamental and applied biological sciences. Psychology
General pharmacology
HPLC–fluorescence detection
Lignans
Lignans - blood
Lignans - pharmacokinetics
Male
Medical sciences
Pharmacokinetic study
Pharmacology. Drug treatments
Phyllanthus - chemistry
Phyllanthus niruri L
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
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Summary:A simple analytical method using HPLC with fluorescence detection was developed for the simultaneous determination of four lignans, phyllanthin ( 1), hypophyllanthin ( 2), phyltetralin ( 3) and niranthin ( 4) from Phyllanthus niruri L. in plasma. The method recorded limits of detection for 1, 2, 3 and 4 as 1.22, 6.02, 0.61 and 1.22 ng/ml, respectively, at a signal-to-noise ratio of 5:1 whereas their limits of quantification were 4.88, 24.41, 4.88 and 9.76 ng/ml, respectively, at a signal-to-noise ratio of 12:1. These values were comparable to those of other sensitive methods such as gas chromatography–mass spectrometry (GC–MS), high-performance liquid chromatography–MS (HPLC–MS) and HPLC–electrochemical detection (HPLC–ECD) for the analysis of plasma lignans. A further advantage over known methods was its simple protocol for sample preparation. The within-day and between-day accuracies for the analysis of the four lignans were between 87.69 and 110.07% with precision values below 10.51%. Their mean recoveries from extraction were between 91.39 and 114.67%. The method was successfully applied in the pharmacokinetic study of lignans in rats. Following intravenous administration, the lignans were eliminated slowly from the body with a mean clearance of 0.04, 0.01, 0.03 and 0.02 l/kg h and a mean half-life of 3.56, 3.87, 3.35 and 4.40 h for 1, 2, 3 and 4, respectively. Their peak plasma concentration upon oral administration was 0.18, 0.56, 0.12 and 0.62 μg/ml, respectively, after 1 h. However, their absorption was incomplete with a calculated absolute oral bioavailability of 0.62, 1.52, 4.01 and 2.66% for 1, 2, 3 and 4, respectively.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2007.01.014