RGD-Dependent Mechanotransduction of Suspension Cultured Taxus Cell in Response to Shear Stress

Plant cells cultured in bioreactors are strongly influenced by mechanical forces. However, the molecular mechanism of plant cell mechanoreception has maintained unclear. In animal cells, the Arg‐Gly‐Asp (RGD) motif can be found in proteins of the extracellular matrix. Integrins link the intracellula...

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Bibliographic Details
Published in:Biotechnology progress 2007-05, Vol.23 (3), p.673-679
Main Authors: Gao, H, Gong, Y.W, Yuan, Y.J
Format: Article
Language:English
Subjects:
Arabidopsis
Biological and medical sciences
bioreactors
biosynthesis
Biotechnology
Blotting, Western
cell suspension culture
Cells, Cultured
Chromatography, Liquid
extracellular matrix
Fundamental and applied biological sciences. Psychology
integrins
mechanotransduction
Mitogen-Activated Protein Kinases - metabolism
Oligopeptides - pharmacology
phenolic compounds
Phenols - metabolism
plant proteins
protein kinases
protein phosphorylation
reactive oxygen species
Reactive Oxygen Species - metabolism
RGD peptide
shear stress
signal transduction
Signal Transduction - drug effects
Stress, Mechanical
synthetic peptides
Tandem Mass Spectrometry
Taxus
Taxus - cytology
Taxus - drug effects
Taxus - metabolism
Taxus cuspidata
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Summary:Plant cells cultured in bioreactors are strongly influenced by mechanical forces. However, the molecular mechanism of plant cell mechanoreception has maintained unclear. In animal cells, the Arg‐Gly‐Asp (RGD) motif can be found in proteins of the extracellular matrix. Integrins link the intracellular cytoskeleton of cells with the extracellular matrix by recognizing this RGD motif. Integrin has been demonstrated to function as an apparatus not only for adhesion but also for mechanotransduction. In plant cells, the molecules that mediate the structural continuity between wall and membrane are unknown. Here, we found that synthetic RGD peptide could dramatically reduce the level of phosphorylation of MAPK‐like cascades that are activated by shear stress and reduce the alkalinization response, production of reactive oxygen species (ROS) and accumulation of phenolics by Taxus cuspidata cells during shear stress. These results implicate that a RGD recognition system may exist in Taxus cells and play an important role in signal transduction of shear stress. Although the Arabidopsis genome database shows that the plant seems to lack a homologue of animal integrin, plant cells may use other RGD‐binding proteins to recognize the RGD motif. The correlative mechanism is discussed.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp060329+