Microsatellite instability at AAAG repeat sequences in respiratory tract cancers

We surveyed the occurrence of novel alleles at microsatellite sequences in non‐small cell lung cancers (NSCLC) using 61 tetranucleotide repeat markers. The presence of at least one new allele, consistent with microsatellite instability (MSI), was observed in 26 of 61 (43%) markers involving 30 of 47...

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Bibliographic Details
Published in:International journal of cancer 2001-01, Vol.91 (2), p.200-204
Main Authors: Xu, LiHua, Chow, John, Bonacum, Julie, Eisenberger, Claus, Ahrendt, Steve A., Spafford, Michael, Wu, Li, Lee, Sheng M., Piantadosi, Steven, Tockman, Melvyn S., Sidransky, David, Jen, Jin
Format: Article
Language:English
Subjects:
AAAG repeats
Aged
Aged, 80 and over
Biological and medical sciences
cancer genetics
Carcinoma, Non-Small-Cell Lung - genetics
Female
Humans
Lung Neoplasms - genetics
Male
Medical sciences
Microsatellite instability
Microsatellite Repeats
Middle Aged
NSCLC
Organ Specificity
Pneumology
Tumor detection
Tumors of the respiratory system and mediastinum
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Summary:We surveyed the occurrence of novel alleles at microsatellite sequences in non‐small cell lung cancers (NSCLC) using 61 tetranucleotide repeat markers. The presence of at least one new allele, consistent with microsatellite instability (MSI), was observed in 26 of 61 (43%) markers involving 30 of 47 (64%) NSCLC. Twelve of the 26 markers detected new alleles in 2 or more tumors and 11 of these 12 markers contained an AAAG repeat sequence. Using this panel of 12 markers, MSI was detected in 24 of 47 (51%) NSCLC and 10 of 18 (56%) head and neck cancers but was only observed in 8 of 38 (21%) bladder cancers and 3 of 25 (12%) kidney cancers. Our results suggested that about 50% of respiratory tract cancers exhibited microsatellite instability predominantly at AAAG sequences. This distinct type of instability was termed EMAST for elevated microsatellite alterations at selected tetranucleotide repeats. The identification of markers with EMAST should have potential application for the molecular detection of respiratory tract cancers. © 2001 Wiley‐Liss, Inc.
ISSN:0020-7136
1097-0215
DOI:10.1002/1097-0215(200002)9999:9999<::AID-IJC1031>3.0.CO;2-0