Not growth associated protein GAP-43 (B-50), but its fragment GAP-43-3 (B-60) predominates in rat brain during development

The participation of the nerve termini growth associated protein GAP-43 in neurite outgrowth and targeting is well documented. Commonly, besides GAP-43 itself, two big fragments devoid of four (GAP-43-2, IB-50) and of about 40 (GAP-43-3, B-60) N-terminal residues were co-isolated from brain. In adul...

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Bibliographic Details
Published in:Neuroscience letters 2001-01, Vol.297 (1), p.49-52
Main Authors: Mosevitsky, Mark I, Konovalova, Ekaterina S, Bitchevaya, Natalja K, Klementiev, Boris I
Format: Article
Language:eng ; rus
Subjects:
Animals
Animals, Newborn
Brain - embryology
Brain - growth & development
Brain - metabolism
Female
GAP-43 Protein - metabolism
Nerve growth associated protein-43/B-50
Neuroontogenesis
Pregnancy
Protein Isoforms - metabolism
Rats
Rats, Wistar
Site-specific proteolysis
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Summary:The participation of the nerve termini growth associated protein GAP-43 in neurite outgrowth and targeting is well documented. Commonly, besides GAP-43 itself, two big fragments devoid of four (GAP-43-2, IB-50) and of about 40 (GAP-43-3, B-60) N-terminal residues were co-isolated from brain. In adult brain, GAP-43 significantly prevails over the fragments. To find their relative amounts during development, rat brain proteins were isolated on different stages of embryonal and post-natal development and subjected to gel electrophoresis in 0.9 M acetic acid-2.5 M urea system. The bands of GAP-43 protein family were detected on Western blots. We show that in developing brain (until 5th post-natal day), a proteolysis of GAP-43 near Ser 41 that results in GAP-43-3 accumulation is activated. We hypothesize that just the functions that can be performed by the GAP-43 fragments are of importance for developing brain.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(00)01666-9