The production of rhamnolipid by a Pseudomonas aeruginosa strain isolated from a southern coastal zone in Brazil

The production and properties of a rhamnolipid-type biosurfactant, synthesized by the Pseudomonas aeruginosa LBM10 strain, isolated from a southern coastal zone in Brazil, were investigated. The assays were conducted in a rotary shaker at 30 °C and 180 rpm for a period of 96 h. Soybean oil and sodiu...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2008-04, Vol.71 (9), p.1781-1785
Main Authors: Prieto, L.M., Michelon, M., Burkert, J.F.M., Kalil, S.J., Burkert, C.A.V.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosurfactants
Biotechnology
Brazil
Carbon - metabolism
Emulsions
Fundamental and applied biological sciences. Psychology
Glycolipids
Hydrocarbon emulsification
Hydrogen-Ion Concentration
Lipids - biosynthesis
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Nitrogen - metabolism
Nitrogen limitation
Pseudomonas aeruginosa
Pseudomonas aeruginosa - metabolism
Surface-active compounds
Temperature
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Summary:The production and properties of a rhamnolipid-type biosurfactant, synthesized by the Pseudomonas aeruginosa LBM10 strain, isolated from a southern coastal zone in Brazil, were investigated. The assays were conducted in a rotary shaker at 30 °C and 180 rpm for a period of 96 h. Soybean oil and sodium nitrate were the best sources of carbon and nitrogen, respectively. A nitrogen-limiting condition (C/N ratio of 100) was favorable to biosurfactant production. The formation of stable emulsions was better in saline concentrations below 0.5%, pH values in the range from 6 to 9 and temperatures in the range from 35 to 40 °C, maintaining about 80% of its original activity for salinity up to 3% and 120 min of exposure at 100 °C. The biosurfactant may be produced with this microorganism using renewable substrates that are readily available, reaching values of 1.42 g l −1 measured as rhamnose. This biosurfactant has interesting and useful properties for many industrial applications.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2008.01.003