Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli

Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by E...

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Bibliographic Details
Published in:FEMS microbiology letters 2007-06, Vol.271 (1), p.126-135
Main Authors: Ooka, Tadasuke, Vieira, Mônica A.M, Ogura, Yoshitoshi, Beutin, Lothar, La Ragione, Roberto, van Diemen, Pauline M, Stevens, Mark P, Aktan, Ilknur, Cawthraw, Shaun, Best, Angus, Hernandes, Rodrigo T, Krause, Gladys, Gomes, Tania A.T, Hayashi, Tetsuya, Frankel, Gad
Format: Article
Language:English
Subjects:
Actin
Amino Acid Sequence
Animals
Animals, Domestic
Bacteria
Bacteriology
Biological and medical sciences
Colonization
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Domestic animals
E coli
Enterocytes
EPEC
Escherichia coli
Escherichia coli - genetics
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Escherichia coli Proteins - biosynthesis
Escherichia coli Proteins - chemistry
Escherichia coli Proteins - genetics
Fundamental and applied biological sciences. Psychology
Humans
Intestine
Microbiology
Molecular Sequence Data
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phosphorylation
Phylogeny
Polymerase Chain Reaction
Secretion
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Signaling
TccP
Tir gene
Translocation
type III secretion
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Summary:Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the translocation of the type III secretion system effector Tir into enterocytes, which following phosphorylation (Tir-Yp) recruits Nck to activate the N-WASP actin signalling cascade. The authors have recently shown that typical EPEC strains, belonging to the EPEC-2 lineage, carry a tir gene encoding Tir-Yp and can also use the alternative TccP2 actin-signalling cascade. The aim of this study was to determine if tccP2 is found in atypical EPEC isolated from human and farm animals. tccP2 was found at a frequency of 41% in non-O26 EPEC isolates and in 82.3% of the O26 strains. TccP2 of human and animal strains show high level of sequence identity. It is shown that most strains carry a tir gene encoding Tir-Yp. In addition the authors identified two new variants of tir genes in EPEC O104:H12 and NT:H19 strains.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2007.00707.x