Recombinant heat shock protein 65 carrying hepatitis B core antigen induces HBcAg-specific CTL response

Abstract Many studies have provided evidence that heat shock protein 65 (Hsp65) can elicit potent specific cellular adaptive immune responses (e.g. CD8+ cytotoxic T-cell effectors or classic CTLs) based on their ability to chaperone antigenic peptides. Hsp65 is thus an effective carrier for heterolo...

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Bibliographic Details
Published in:Vaccine 2007-05, Vol.25 (22), p.4478-4486
Main Authors: Yang, Bing-fen, Zhao, Hong-liang, Xue, Chong, Xiong, Xiang-hua, Zhang, Wei, Yao, Xue-qin, Liu, Zhi-min
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antigen Presentation
Antigens
Applied microbiology
Bacterial Proteins - genetics
Bacterial Proteins - immunology
Bacterial Proteins - metabolism
Biological and medical sciences
Chaperonin 60
Chaperonins - genetics
Chaperonins - immunology
Chaperonins - metabolism
Chronic hepatitis B virus infection
Core antigen of hepatitis B virus (HBcAg)
Cytotoxicity
Deoxyribonucleic acid
DNA
DNA polymerase
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
Genes
Heat shock protein 65
Heat shock proteins
Hepatitis
Hepatitis B - immunology
Hepatitis B Antibodies - blood
Hepatitis B Core Antigens - genetics
Hepatitis B Core Antigens - immunology
Hepatitis B Core Antigens - metabolism
Hepatitis B Vaccines - administration & dosage
Hepatitis B Vaccines - genetics
Hepatitis B Vaccines - immunology
Hepatitis B virus
Hepatitis B, Chronic - immunology
Hepatitis B, Chronic - prevention & control
Hepatitis B, Chronic - virology
Human viral diseases
Humans
Infections
Infectious diseases
Medical sciences
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Peptides
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - metabolism
T cell receptors
T-Lymphocytes, Cytotoxic - immunology
Therapeutic vaccine
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Viral diseases
Viral hepatitis
Virology
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Summary:Abstract Many studies have provided evidence that heat shock protein 65 (Hsp65) can elicit potent specific cellular adaptive immune responses (e.g. CD8+ cytotoxic T-cell effectors or classic CTLs) based on their ability to chaperone antigenic peptides. Hsp65 is thus an effective carrier for heterologous peptide epitopes for therapeutic vaccines against cancer or chronic infectious diseases. The core antigen of hepatitis B virus (HBcAg) is extremely immunogenic, and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. Here, a chimeric protein, Hsp65Bc, was created by fusing the HBcAg sequence to the carboxyl terminus of the Hsp65 sequence in E. coli . Analysis of its antigenicity and immunogenicity revealed that HBc epitopes are surface accessible. Hsp65Bc induced moderate anti-HBc immune responses as well as a strong specific T-cell response in BALB/c mice. These results indicate that Hsp65Bc may have potential as a vaccine for treatment of HBV chronic infection.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2007.03.020