Dexamethasone-induced Ras-related protein 1 is a potential regulatory protein in B lymphocytes

Dexamethasone-induced Ras-related protein 1 (RASD1) is a protein of the Ras family which probably has a regulatory function. We demonstrate that Rasd1 mRNA is expressed in mouse lymph node cells in response to inhibitory stimuli. Rasd1 mRNA is present at very low levels in freshly isolated cells, bu...

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Bibliographic Details
Published in:International immunology 2007-05, Vol.19 (5), p.583-590
Main Author: Lindsey, JW
Format: Article
Language:English
Subjects:
Animals
B cells
B-Lymphocytes - cytology
B-Lymphocytes - drug effects
B-Lymphocytes - immunology
Cell Proliferation - drug effects
Cells, Cultured
Dexamethasone - pharmacology
Female
Gene Expression Regulation - drug effects
Gene Expression Regulation - immunology
Genes, ras - drug effects
Histocompatibility Antigens Class II - immunology
immune regulation
Lymph Nodes - immunology
Lymphocyte Activation - drug effects
Lymphocyte Activation - immunology
Mice
Ras proteins
ras Proteins - biosynthesis
ras Proteins - drug effects
ras Proteins - immunology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - isolation & purification
Thy-1 Antigens - immunology
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Summary:Dexamethasone-induced Ras-related protein 1 (RASD1) is a protein of the Ras family which probably has a regulatory function. We demonstrate that Rasd1 mRNA is expressed in mouse lymph node cells in response to inhibitory stimuli. Rasd1 mRNA is present at very low levels in freshly isolated cells, but it is rapidly up-regulated in culture and is expressed at elevated levels in cells whose proliferation is blocked by exposure to homogenized brain tissue. The cells expressing Rasd1 mRNA are positive for MHC class II and B220 and negative for Thy-1. Expression of Rasd1 mRNA is very low in B cell-deficient mice. We conclude that Rasd1 mRNA is expressed by B lymphocytes derived from lymph node cells in response to inactivating or inhibitory stimuli. It may play a role in regulating B lymphocyte activity and proliferation.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/dxm023