Isolation and identification of equol-producing bacterial strains from cultures of pig faeces

Transformation of daidzein to equol was compared during fermentation of three growth media inoculated with faeces from Erhualian piglets, but equol was produced from only one medium, M1. Two equol-producing strains (D1 and D2) were subsequently isolated using medium M1. Both strains were identified...

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Bibliographic Details
Published in:FEMS microbiology letters 2008-05, Vol.282 (1), p.73-80
Main Authors: Yu, Zhuo-Teng, Yao, Wen, Zhu, Wei-Yun
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Biological and medical sciences
China
Culture Media - chemistry
Daidzein
daidzein transformation
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Equol
equol production
Feces
Feces - microbiology
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic transformation
Growth media
Intestine
Isoflavones - analysis
Isoflavones - metabolism
Microbiology
Molecular Sequence Data
Phylogeny
Physical characteristics
pig faecal bacteria
RNA, Ribosomal, 16S - genetics
rRNA 16S
Strains (organisms)
Swine - microbiology
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Summary:Transformation of daidzein to equol was compared during fermentation of three growth media inoculated with faeces from Erhualian piglets, but equol was produced from only one medium, M1. Two equol-producing strains (D1 and D2) were subsequently isolated using medium M1. Both strains were identified as Eubacterium sp., on the basis of morphological and physiological characteristics, and 16S rRNA gene sequence analysis showed that strains D1 and D2 were most closely related to previously characterized daidzein-metabolizing bacteria isolated from human faecal and rumen samples, respectively. This suggests that the ability to metabolize daidzein can be found among bacteria present within the mammalian intestine. The results provided the first account of conversion of daidzein directly to equol by bacterial species from farm animals. These strains may be of importance to the improvement of animal performance, and the use of medium M1 could provide a simple way to isolate bacterial strains capable of transforming daidzein into equol.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2008.01108.x