Synthetic glycolipid modification of red blood cell membranes

BACKGROUND: Glycolipids have a natural ability to insert into red cell (RBC) membranes. Based on this concept the serology of RBCs modified with synthetic analogs of blood group glycolipids (KODE technology) was developed, which entails making synthetic glycolipid constructs engineered to have speci...

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Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2007-05, Vol.47 (5), p.876-882
Main Authors: Frame, Tom, Carroll, Tim, Korchagina, Elena, Bovin, Nicolai, Henry, Stephen
Format: Article
Language:English
Subjects:
ABO Blood-Group System - immunology
Antibodies, Monoclonal - immunology
Antibody Specificity
Erythrocyte Membrane - chemistry
Erythrocytes - chemistry
Erythrocytes - immunology
Glycolipids - chemical synthesis
Glycolipids - chemistry
Glycolipids - immunology
Humans
Lewis Blood-Group System - immunology
Membrane Lipids - chemical synthesis
Membrane Lipids - chemistry
Membrane Lipids - immunology
Microscopy, Fluorescence
Molecular Structure
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Summary:BACKGROUND: Glycolipids have a natural ability to insert into red cell (RBC) membranes. Based on this concept the serology of RBCs modified with synthetic analogs of blood group glycolipids (KODE technology) was developed, which entails making synthetic glycolipid constructs engineered to have specific performance criteria. Such synthetic constructs can be made to express a potentially unlimited range of carbohydrate blood group determinants. STUDY DESIGN AND METHODS: Synthetic constructs incorporating A, B, acquired‐B, and Lea blood group determinants were constructed and used to modify RBCs. Modified cells were assessed by routine serologic methods using a range of commercially available monoclonal antibodies. RESULTS: RBCs modified with different concentrations of synthetic glycolipids were able to give controllable serologic results. Synthetic A and B modified cells were able to be created to represent the serology of “weak” subgroups. Specialized cells such as those bearing synthetic acquired‐B antigen reacted as expected, but also exhibited extended features due to the cells bearing only specific antigen. Synthetic Lea‐modified cells reacted as expected with anti‐Lea reagents, but unexpectedly, were also able to detect the chemical anti‐Leab specificity of serologic monoclonal anti‐Leb reagents. CONCLUSION: RBCs can be created to express normal and novel carbohydrate profiles by inserting synthetic glycolipids into them. Such cells will be useful in creating specialized antigen panels and for quality control purposes.
ISSN:0041-1132
1537-2995
DOI:10.1111/j.1537-2995.2007.01204.x