Apolipoprotein A-IMilano /POPC complex attenuates post-ischemic ventricular dysfunction in the isolated rabbit heart

Abstract Irreversible myocardial injury is a potential consequence of coronary artery revascularization. Reperfusion leads to the production of oxidized products that can damage myocardium. High-density lipoproteins (HDL) are effective at removing oxidized lipids. We hypothesized that a synthetic HD...

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Bibliographic Details
Published in:Atherosclerosis 2008-04, Vol.197 (2), p.572-578
Main Authors: Marchesi, Marta, Booth, Erin A, Rossoni, Giuseppe, García, Ricardo A, Hill, Knut R, Sirtori, Cesare R, Bisgaier, Charles L, Lucchesi, Benedict R
Format: Article
Language:English
Subjects:
Animals
Antioxidant
Antioxidants - pharmacology
Apolipoprotein A-I
Apolipoprotein A-I - pharmacology
Apolipoprotein A-IMilano
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cardiovascular
Disease Models, Animal
High-density lipoproteins
Ischemia reperfusion injury
Lipid Peroxidation - drug effects
Lipoproteins, HDL - pharmacology
Male
Medical sciences
Microscopy, Electron
Myocardial Reperfusion Injury - pathology
Myocardial Reperfusion Injury - prevention & control
Phosphatidylcholines - pharmacology
Rabbits
Recombinant Proteins - pharmacology
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels
Ventricular Dysfunction - pathology
Ventricular Dysfunction - prevention & control
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Summary:Abstract Irreversible myocardial injury is a potential consequence of coronary artery revascularization. Reperfusion leads to the production of oxidized products that can damage myocardium. High-density lipoproteins (HDL) are effective at removing oxidized lipids. We hypothesized that a synthetic HDL preparation, comprising recombinant apolipoprotein A-IMilano (apoA-IM ) complexed with 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC) (apoA-IM /POPC) would protect the heart from reperfusion injury. The ex vivo model consisted of rabbit hearts perfused by the Langendorff method. Hearts were equilibrated with Krebs–Henseleit buffer (10 min), pretreated with either apoA-IM /POPC (0.45 mg/mL) or vehicle (10 min), subjected to global ischemia (30 min) and reperfused for 60 min. ApoA-IM /POPC ( n = 7) prevented the left ventricular end-diastolic pressure elevation observed in the vehicle group ( n = 6) at the end of reperfusion ( p < 0.05). During reperfusion, coronary artery perfusion pressure increased in the controls ( p < 0.001), but not with apoA-IM /POPC. ApoA-IM /POPC reduced the release of creatine kinase at the end of the ischemic period ( p < 0.001). It also reduced cardiac left ventricle muscle lipid hydroperoxides by 46% ( p < 0.05). Direct comparison of the antioxidant potential indicated that recombinant apoA-IM was much more potent than apoA-I in attenuating low-density lipoprotein oxidation. Electron microscopy showed that apoA-IM /POPC prevented mitochondrial granulation, disorganization and sarcomere contraction band formation indicative of reperfusion injury. The apoA-IM /POPC complex thus appears to reduce reperfusion injury under global ischemic conditions, and may therefore have therapeutic application in the reduction of myocardial ischemia.
ISSN:0021-9150
1879-1484
DOI:10.1016/j.atherosclerosis.2007.08.028