Bovine dialyzable leukocyte extract modulates AP-1 DNA-binding activity and nuclear transcription factor expression in MCF-7 breast cancer cells

Background We have previously demonstrated that bovine dialyzable leukocyte extract (bDLE) induces death through an apoptosis mechanism in MCF-7 breast cancer cells. Depending on the cell type and stimulus, activating protein-1 (AP-1) has been shown to regulate cell proliferation and differentiation...

Full description

Saved in:
Bibliographic Details
Published in:Cytotherapy (Oxford, England) England), 2008-01, Vol.10 (2), p.212-219
Main Authors: Mendoza-Gamboa, E, Franco-Molina, M.A, Zapata-Benavides, P, Castillo-Tello, P, Vera-García, M.E, Tamez-Guerra, R.S, Rodríguez-Padilla, C
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
AP-1
bovine dialyzable leukocyte extract
breast cancer cells
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cattle
Cell Line, Tumor
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cell Proliferation - drug effects
DNA, Neoplasm - metabolism
Female
Gene Expression Regulation, Neoplastic - drug effects
Humans
Other
Protein Binding - drug effects
Proto-Oncogene Proteins c-fos - genetics
Proto-Oncogene Proteins c-fos - metabolism
Transcription Factor AP-1 - genetics
Transcription Factor AP-1 - metabolism
Transfer Factor - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background We have previously demonstrated that bovine dialyzable leukocyte extract (bDLE) induces death through an apoptosis mechanism in MCF-7 breast cancer cells. Depending on the cell type and stimulus, activating protein-1 (AP-1) has been shown to regulate cell proliferation and differentiation, the stress response, apoptosis and survival. It remains unknown whether AP-1 and other transcription factors are mechanisms by which bDLE induces cell death. Methods To determine whether bDLE modulates the AP-1 DNA binding and gene expression, MCF-7 breast cancer cells were treated with bDLE (0, 1, 5, 10 U) for 72 h and evaluated by electrophoretic mobility shift assay, reverse transcriptase-polymerase chain reaction and Western blot assays. Results bDLE induced inhibition of cell growth, suppressed the AP-1 DNA-binding activity, decreased c-Jun protein expression and modulated NFATx, NFATc, NFκB, c-Jun and c-Fos transcription factor gene expression in MCF-7 breast cancer cells. Discussion The present data indicate that bDLE can block the AP-1 DNA-binding activity and expression of several transcriptions factors in breast cancer cells, which will have great potential in improving cancer therapy.
ISSN:1465-3249
1477-2566
DOI:10.1080/14653240801891659