A rapid fluorimetric screening method for the 1,4-benzodiazepines: Determination of their metabolite oxazepam in urine

Oxazepam is the major metabolite screened in urine samples for the evidence of the use of benzodiazepine drugs. The methods currently used, however, are laborious and time consuming. This paper proposes an oxazepam detection method based on its hydrolysis and cyclization – a reaction catalysed by ce...

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Bibliographic Details
Published in:Analytica chimica acta 2007-05, Vol.591 (1), p.112-115
Main Authors: Gil Tejedor, A.M., Fernández Hernando, P., Durand Alegría, J.S.
Format: Article
Language:English
Subjects:
Analytical chemistry
Benzodiazepines
Chemistry
Clorazepate Dipotassium - metabolism
Clorazepate Dipotassium - urine
Diazepam - metabolism
Diazepam - urine
Exact sciences and technology
Fluorimetry
Humans
Oxazepam
Oxazepam - urine
Screening
Sensitivity and Specificity
Spectrometric and optical methods
Spectrometry, Fluorescence
Urine
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Summary:Oxazepam is the major metabolite screened in urine samples for the evidence of the use of benzodiazepine drugs. The methods currently used, however, are laborious and time consuming. This paper proposes an oxazepam detection method based on its hydrolysis and cyclization – a reaction catalysed by cerium (IV) in an ortho-phosphoric acid-containing medium – to form 2-chloro-9(10H)-acridinone, a strongly fluorescent molecule. The variables involved in the hydrolysis and cyclization stages were optimised. Oxazepam was detectable in the 5–900 ng mL −1 range, with a detection limit of 4.15 ng mL −1 for k = 3. The method was successfully used for the determination of oxazepam in urine samples collected at different times after the oral administration of Valium ® and Tranxilium ®.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2007.03.055