Arg-gingipain A DNA Vaccine Prevents Alveolar Bone Loss in Mice

One major pathogenic factor of Porphyromonas gingivalis is Arg-gingipain (Rgp), an arginine-specific cysteine proteinase. To clarify the effect of rgpA DNA vaccine, we immunized BALB/c mice via the abdomen with a Gene Gun or via the nasal cavity weekly for 6 weeks. After immunization, the mice were...

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Bibliographic Details
Published in:Journal of dental research 2007-05, Vol.86 (5), p.446-450
Main Authors: Miyachi, K., Ishihara, K., Kimizuka, R., Okuda, K.
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - immunology
Administration, Intranasal
Alveolar Bone Loss - microbiology
Alveolar Bone Loss - prevention & control
Analysis of Variance
Animals
Antibodies, Bacterial - biosynthesis
Bacterial Vaccines - administration & dosage
Bacterial Vaccines - chemistry
Bacterial Vaccines - therapeutic use
Biolistics
Cysteine Endopeptidases - immunology
Dentistry
Female
Mice
Mice, Inbred BALB C
Porphyromonas gingivalis
Porphyromonas gingivalis - enzymology
Porphyromonas gingivalis - immunology
Recombinant Proteins
Statistics, Nonparametric
Vaccines, DNA - administration & dosage
Vaccines, DNA - therapeutic use
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Summary:One major pathogenic factor of Porphyromonas gingivalis is Arg-gingipain (Rgp), an arginine-specific cysteine proteinase. To clarify the effect of rgpA DNA vaccine, we immunized BALB/c mice via the abdomen with a Gene Gun or via the nasal cavity weekly for 6 weeks. After immunization, the mice were challenged orally with P. gingivalis. Immunization elicited IgG responses against P. gingivalis in both groups. Nasal immunization also induced sIgA against P. gingivalis, although Gene Gun immunization did not. Reduction of alveolar bone loss was observed in both groups at 42 days following initial infection. This effect was more pronounced in the intranasal immunization group than in the Gene Gun group. The results of this study suggest that immunization with rgpA DNA vaccine via the nasal cavity is an effective method for preventing alveolar bone loss incurred by infection with P. gingivalis.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910708600511