Microarray Analysis of BeWo and JEG3 Trophoblast Cell Lines: Identification of Differentially Expressed Transcripts

Abstract Trophoblast cell lines are important research tools used as a surrogate for primary trophoblast cells in the study of placental function. Because the cellular origins of transformed trophoblasts are likely to be diverse, it would be of value to understand the unique and shared phenotypes of...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2007-05, Vol.28 (5), p.383-389
Main Authors: Burleigh, D.W, Kendziorski, C.M, Choi, Y.J, Grindle, K.M, Grendell, R.L, Magness, R.R, Golos, T.G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Line
DNA Primers
Embryology: invertebrates and vertebrates. Teratology
Female
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental
Humans
Integrins - genetics
Internal Medicine
Obstetrics and Gynecology
Oligonucleotide Array Sequence Analysis
Placenta - cytology
Placenta - physiology
Polymerase Chain Reaction
Pregnancy
Proteins - genetics
Transcription, Genetic
Trophoblasts - cytology
Trophoblasts - physiology
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Summary:Abstract Trophoblast cell lines are important research tools used as a surrogate for primary trophoblast cells in the study of placental function. Because the cellular origins of transformed trophoblasts are likely to be diverse, it would be of value to understand the unique and shared phenotypes of the cells on a global scale. We have compared two widely used cell lines, BeWo and JEG3, by microarray analysis in order to identify differentially expressed genes. Results indicated that approximately 2700 genes were differentially expressed between the cell lines, with principal differences observed in the biological processes of response to stress, cell adhesion, signal transduction, and protein and nucleobase metabolisms. These data suggest that BeWo and JEG3 cell lines, and perhaps other trophoblast cell lines, are sufficiently dissimilar from each other such that they will be differentially suited for specific experimental paradigms.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2006.05.001