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Flowcytometric quantitation of hepatitis B viral antigens in hepatocytes from regular and fine-needle biopsies

The aim of the study was to investigate the use of flow cytometry, as an alternative for immunohistochemistry, for the detection of viral antigens in the liver of patients with chronic hepatitis B virus (HBV) infection. Hepatocytes were obtained from regular- and fine-needle biopsy from HBV positive...

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Bibliographic Details
Published in:Journal of virological methods 2007-06, Vol.142 (1), p.189-197
Main Authors: van der Laan, Luc J.W., Taimr, Pavel, Kok, Alice, Sprengers, Dave, Zondervan, Pieter E., Tilanus, Hugo W., Janssen, Harry L.A.
Format: Article
Language:English
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Summary:The aim of the study was to investigate the use of flow cytometry, as an alternative for immunohistochemistry, for the detection of viral antigens in the liver of patients with chronic hepatitis B virus (HBV) infection. Hepatocytes were obtained from regular- and fine-needle biopsy from HBV positive ( n = 17) and negative ( n = 7) patients and quantified by flow cytometry for intracellular hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg). Number of HBsAg positive hepatocytes ranged from 0 to 83%. A significant correlation was found between the percentage of infected hepatocytes and the intracellular expression level of HBsAg ( R = 0.841, p < 0.001). The specificity and sensitivity of flow cytometry was similar to immunohistochemistry. Of the patients on anti-viral treatment with undetectable serum HBV DNA (
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2007.01.027