Development of NaCl-tolerant line in Chrysanthemum morifolium Ramat. through shoot organogenesis of selected callus line

Plants were regenerated successfully through shoot organogenesis of a NaCl-selected callus line of Chrysanthemum morifolium Ramat. cv. Maghi Yellow (a salt sensitive cultivar), developed through stepwise increase in NaCl concentration (0–100 mM) in the MS medium. The stepwise increase in NaCl concen...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biotechnology 2007-05, Vol.129 (4), p.658-667
Main Authors: Hossain, Zahed, Mandal, Abul Kalam Azad, Datta, Subodh Kumar, Biswas, Amal Krishna
Format: Article
Language:English
Subjects:
Antioxidant enzymes
Ascorbate Peroxidases
Biological and medical sciences
Biotechnology
callus
callus culture
Cell Culture Techniques
Chrysanthemum
Chrysanthemum - classification
Chrysanthemum - drug effects
Chrysanthemum - enzymology
Chrysanthemum - physiology
Chrysanthemum morifolium
Drug Tolerance
Fundamental and applied biological sciences. Psychology
Glutathione Reductase - metabolism
In vitro
in vitro regeneration
NaCl-tolerant callus line
organogenesis
Peroxidases - metabolism
Proline - metabolism
Regeneration
ROS
Salinity stress
salt stress
salt tolerance
shoots
sodium chloride
Sodium Chloride - pharmacology
Superoxide Dismutase - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plants were regenerated successfully through shoot organogenesis of a NaCl-selected callus line of Chrysanthemum morifolium Ramat. cv. Maghi Yellow (a salt sensitive cultivar), developed through stepwise increase in NaCl concentration (0–100 mM) in the MS medium. The stepwise increase in NaCl concentration from a relatively low level to cytotoxic level was found to be a better way to isolate NaCl-tolerant callus line, since direct transfer of callus to high saline medium was detrimental to callus survival and growth. The selected callus line exhibited significant increase in superoxide dismutase (EC 1.15.1.1), ascorbate peroxidase (EC 1.11.1.11) and glutathione reductase (EC 1.6.4.2) activities compared to control callus (grown in medium devoid of NaCl). Stability of salt tolerance character of the selected callus line was checked by growing the calli in NaCl-free medium for 3 consecutive months followed by re-exposure to higher salinity stress (120 mM NaCl). Among different growth regulator treatments, a combination of 5 mg l −1 TDZ (Thidiazuron) along with 0.25 mg l −1 NAA and 0.5 mg l −1 GA 3 was found to be the most effective for shoot organogenesis in selected callus line. The regeneration potential of the NaCl-tolerant callus ranged from 20.8% to 0% against 62.4% to 0% in control callus line. Under elevated stress condition (medium supplemented with 250 mM NaCl), selected calli derived regenerants (S1 plants) exhibited significantly higher SOD and APX activities over both PC (positive control: control callus derived plants grown on MS medium devoid of NaCl) and NC (negative control: control callus derived plants subjected to 250 mM NaCl stress) plants. In addition, the NC plants showed stunted growth, delayed root initiation, and had lesser number of roots as compared to S1 plants. Based on growth performance and antioxidant capacity, the S1 plants could be considered as NaCl-tolerant line showing all positive adaptive features towards the salinity stress. Further study on agronomic performance of these S1 plants under saline soil condition need to be undertaken to check the genetic stability of the induced salt-tolerance.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2007.02.020